BIMM110      LECTURES 10 - 11

TRANSGENIC ANIMALS KNOCK-OUT MICE

CLONING OF ANIMALS

SLIDES

Textbook: Strachan and Read, Chapters 20 & 21

1. Gene transfer into animal cells in tissue culture
2. Gene transfer into embryonic stem cells or oocytes
3. Gene knock-outs in mouse embryonic stem cells

Hello Dolly

- cloning of mammals
- fusion of enucleated secondary oocyte with nucleated somatic cell from selected donor
- note that the resulting individual will have most if not all of its mitochondrial DNA from the oocyte donor - it will not be a true clone
Cloning
In Vitro Fertilization (San Diego Medical Group)