Physical Biochemistry - BIBC 110 -Summer 2005

Physical Biochemistry - Summer 05

Dr. Lukas Buehler

Office Hours MW 3:30-4:30 PM Bonner Hall 2238

Course Outline (download syllabus in pdf format)

This course introduces the principles of physical biochemistry and its applications to the study of macromolecular structure and function. The course covers thermodynamic principles in understanding behavior of macromolecules such as solubility and enzymatic activity. Structure determination techniques are presented to show the experimental approaches addressing structure function relationship of proteins at different levels of resolutions, both conformational and in time. Finally, surface active molecules and biological membranes are discussed including chemical equilibrium phenomena across membranes, self assembly properties of membranes and channel forming peptides and transport processes vital for the function of biological activity in the brain.

Updates
last update 9/6/05

The grades are in and should appear on your transcript by the end of the week.

The exams and reports are at York Hall 3080. You will find the grade for the exam (in red), and grades for presentation, reports, and your overall grade (class grade).

Contact me at lbuehler@ucsd.edu with any questions.

Study guide with answers

Group presentation schedule How-to-guide for presentations and report writing
Monday 8/29	Adrienne Peetz Jason Poulos Stephen Chang	Force as a probe of membrane structure and function
	Christine Tran Sarah Horng Adam Qwan	Single molecule enzymology of chymotrypsin using water-in-oil emulsion
	Michael Kendall Michael Pham	Imaging through fluorescent protein application
Wednesday 8/31	Mohammed Maktabi John Flores	Development of fluorescent protein markers in living cells
	Matt Campbell Tina Cho Kristiana Nguyen	Kinesin moves by an asymmetric hand-over-hand mechanism
	Andrea Herold Melanie Hanan Andrew Radin	Myosin moves along actin filaments in regular steps
	Emilio Ramos	F1-ATPase rotation using single molecule techniques; Total internal reflection microscopy (TIRM)
	Nhan Lun Lien Vo Blanca Hedrick Lindsay Chapman	Single molecule enzymology

	Course Reader	Lecture Slides (PDF)	Biophysical Society On-line Resources
Part I	What physics can tell us about biology	1. Physics of biological systems 2. Molecular interactions and self-assembly systems 3. Lipid monolayers, bilayers, micelles (polymorphism)	Biophysical Techniques Thermodynamics Hydrodynamic methods Spectroscopy Single Molecule Techniques
Part II	The structure of cell membranes	4. Analyzing protein structures; proteomics techniques 5. Absorption spectroscopy; circular dichroism (CD) 6. Emission spectroscopy; fluorescence, diffusion(3.4Mb)	Molecular Structure & Behavior Structure of membrane proteins Lipids & Membranes
Part III	Membrane transport processes & binding phenomena	7. Functional reconstitution 8. Single molecule behavior 9. Modelling transport processes	Biophysical Mechanisms Electrical behavior of cells Energy Transduction in Membranes Protein Function Membrane Behavior

Text Book (Recommended):

Kensal E van Holde, Curtis Johnson, Pui Shing Ho
Principles of Physical Biochemistry, 2/E
ISBN: 0-13-046427-9; Publisher: Prentice Hall; Copyright: 2006

Comments to: lbuehler@ucsd.edu