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ttp://www.microscopyu.com/smallworld/gallery/contests/2001/2ndexlarge2001.html
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BIPN 140 Cellular Neurobiology
Winter 2006
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Welcome to the official class website for BIPN 140 Cellular Neurobiology.
Please scroll down to view the full content. Replace _at_ by @ in the email addresses.
Key for final (questions by Dr.
Ghosh) can be found here.
We found two minor glitches in Problemset 7. A corrected
version is uploaded now.
Q4: In absence of notch, the cells will assume neuronal
fate.
Q12: SynCAM induces presynaptic assembly.
We have also removed the last question in Problemset 8
since it has not been covered in the lecture.
Additional problem set on last lecture posted.
Extra office hour Flavio (usual location) today Monday
from 6.00 pm to 7.00 pm.
No office hour on Thursday.
All problem sets and answers are posted now.
Date, time, and location of final: Fri 03/24/06
11:30a-2:30p CENTR 109
Answer Key for Midterm
MIDTERM READY FOR PICKUP IN CMG Library 2nd
Floor
If you have not signed the waiver: CMG 101 from 1:30 to
2:30 Mo-Fr
PLEASE HIT THE RELOAD BUTTON
IN ORDER TO VIEW THE MOST RECENTLY UPDATED VERSION OF THIS WEBSITE.
Dr. Scanziani’s lab
has moved to the Center for Molecular Genetics (CMG), Room 213.
Previous years’
exams can be found here.
Study section on Monday is
cancelled due to low attendance.
For those who are
interested, you can find the paper on Ih (single channel recordings) which I
mentioned during section here.
This is your interest and to show you that the techniques you learnt in class
are used in research. The content of this paper (beyond what you learnt in
class) is not part of the midterm.
General information and schedule can be downloaded here.
Lectures
Tuesday and Thursday from 12.30 pm to 1.50 pm.
Center 109
Professors
Dr. Massimo Scanziani
Email: mscanziani_at_ucsd.edu
Phone: (858) 822 3839
Dr. Anirvan Ghosh
Email: aghosh_at_ucsd.edu
Phone: (858) 822 4142
Teaching Assistants
Flavio Frohlich
Email: ffrohlic_at_ucsd.edu
Phone: (858) 822 3840
Office hour: Thursday, 2.00 – 3.00 pm CMG 213
Natalie Shanks
Email: nshanks_at_ucsd.edu
Office hour: Wednesday, 4.00 – 5.00 pm Pacific Hall 1115
Darin Quach
Email: darin.quach_at_gmail.com
Office hour: Tuesday, 10.00 – 11.00 am S&E Library 1st Floor
Discussion Sections
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Day |
Time |
Location |
Instructor |
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Tuesdays |
11:00-11:50 am |
HSS 1305 |
Flavio |
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Wednesdays |
9:00-9:50 am |
Center 218 |
Darin |
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Friday |
4:00-4:50 pm |
Center 205B |
Natalie |
Schedule
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Lectures |
Title |
Purves Chapter |
Instructor |
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Lecture 1 |
Tuesday, January 10, 2006 |
1 |
Dr. M. Scanziani |
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Lecture 2 |
Thursday, January 12, 2006 |
2 |
Dr. M. Scanziani |
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Lecture 3 |
Tuesday, January 17, 2006 |
2,3 |
Dr. M. Scanziani |
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Lecture 4 |
Thursday, January 19, 2006 |
4 |
Dr. M. Scanziani |
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Q&A Lecture |
Tuesday, January 24, 2006 |
Q&A Session |
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Lecture 5 |
Thursday, January 26, 2006 |
4 |
Dr. M. Scanziani |
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Lecture 6 |
Tuesday, January 31, 2006 |
5 |
Dr. M. Scanziani |
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Lecture 7 |
Thursday, February 02, 2006 |
5 |
Dr. M. Scanziani |
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Lecture 8 |
Tuesday, February 07, 2006 |
6 |
Dr. M. Scanziani |
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Lecture 9 |
Thursday, February 09, 2006 |
6,7 |
Dr. M. Scanziani |
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Midterm |
Tuesday, February 14, 2006 |
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Dr. M. Scanziani |
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Lecture 10 |
Thursday, February 16, 2006 |
6,7 |
Dr. M. Scanziani |
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Lecture 11 |
Tuesday, February 21, 2006 |
5 |
Dr. M. Scanziani |
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Lecture 12 |
Thursday, February 23, 2006 |
6,7 |
Dr. M. Scanziani |
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Lecture 13 |
Tuesday, February 28, 2006 |
21 |
Dr. A. Ghosh |
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Lecture 14 |
Thursday, March 02, 2006 |
21 |
Dr. A. Ghosh |
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Lecture 15 |
Tuesday, March 07, 2006 |
22 |
Dr. A. Ghosh |
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Lecture 16 |
Thursday, March 09, 2006 |
22 |
Dr. A. Ghosh |
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Lecture 17 |
Tuesday, March 14, 2006 |
22 |
Dr. A. Ghosh |
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Lecture 18 |
Thursday, March 16, 2006 |
23 |
Dr. A. Ghosh |
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Finals |
Friday, March 24, 2006 |
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Problem Sets
Patch clamp method (from the text book)
A wealth of new information about ion channels resulted from the invention of the patch clamp method in the 1970s. This technique is based on a very simple idea. A glass pipette with a very small opening is used to make tight contact with a tiny area, or patch, of neuronal membrane. After the application of a small amount of suction to the back of the pipette, the seal between pipette and membrane becomes so tight that no ions can flow between the pipette and the membrane. Thus, all the ions that flow when a single ion channel opens must flow into the pipette. The resulting electrical current, though small, can be measured with an ultrasensitive electronic amplifier connected to the pipette. Based on the geometry involved, this arrangement usually is called the cell-attached patch clamp recording method. As with the conventional voltage clamp method, the patch clamp method allows experimental control of the membrane potential to characterize the voltage dependence of membrane currents.
Although the ability to record currents flowing through single ion channels is an important advantage of the cell-attached patch clamp method, minor technical modifications yield still other advantages. For example, if the membrane patch within the pipette is disrupted by briefly applying strong suction, the interior of the pipette becomes continuous with the cytoplasm of the cell. This arrangement allows measurements of electrical potentials and currents from the entire cell and is therefore called the whole-cell recording method. The whole-cell configuration also allows diffusional exchange between the pipette and the cytoplasm, producing a convenient way to inject substances into the interior of a "patched" cell.
Two other variants of the patch clamp method originate from the finding that once a tight seal has formed between the membrane and the glass pipette, small pieces of membrane can be pulled away from the cell without disrupting the seal; this yields a preparation that is free of the complications imposed by the rest of the cell. Simply retracting a pipette that is in the cell-attached configuration causes a small vesicle of membrane to remain attached to the pipette. By exposing the tip of the pipette to air, the vesicle opens to yield a small patch of membrane with its (former) intracellular surface exposed. This arrangement, called the inside-out patch recording configuration, allows the measurement of single-channel currents with the added benefit of making it possible to change the medium to which the intracellular surface of the membrane is exposed. Thus, the inside-out configuration is particularly valuable when studying the influence of intracellular molecules on ion channel function. Alternatively, if the pipette is retracted while it is in the whole-cell configuration, a membrane patch is produced that has its extracellular surface exposed. This arrangement, called the outside-out recording configuration, is optimal for studying how channel activity is influenced by extracellular chemical signals, such as neurotransmitters (see Chapter 7). This range of possible configurations makes the patch clamp method an unusually versatile technique for studies of ion channel function.
Pre-exam review sessions
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Date |
Location |
Time |
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Midterm |
Monday, February 13, 2006 |
Pacific Hall 3500 |
5:30-7:30 pm |
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Final |
Thursday, March 23, 2006 |
Pacific Hall 3500 |
5:00-7:00 pm |
Last update of this website: 1/11/2006 FF