Pelican and Stinger: Insulated P Element Transformation Vectors


Barolo, S., Carver, L. A., and Posakony, J. W. (2000). GFP and b-galactosidase transformation vectors for promoter/enhancer analysis in Drosophila. BioTechniques 29: 726-732. PDF of this article
 

VECTOR MAPS (PDF)

VECTOR SEQUENCES (GenBank Accession Numbers)

pPelican:                  AF242360

pH-Pelican:              AF242361

pGreen Pelican:        AF242362

pGreen H-Pelican:    AF242363

pStinger:                 AF242364

pH-Stinger:             AF242365


Nuclear eGFP expression driven by Stinger and UAS-Stinger.  (A)  Nuclear eGFP expression in cells of a proneural cluster in a Drosophila wing imaginal disc, driven by the E(spl)m4 promoter in Stinger.  (B)  Nuclear eGFPexpression in leg and wing imaginal discs from a transgenic larva containingboth UAS-Stinger and patched-GAL4, which drives GAL4 expressionalong the anterior-posterior boundary.  Dotted white lines show the outlines of the discs.  eGFP expression is restricted to nuclei of GAL4-expressing cells.