Supplemental Materials for:

Khwaja, M., Ma, Q., Saier, M.H.Jr., 2004., "Topological Analysis of Integral Membrane Constituents of Prokaryotic ABC Efflux Systems"





Legends for the Supplemenatry Figures (.doc format)


Figure S1 (.aln format) Multiple alignment of recognizable 4 TMS integral membrane protein homologues of the ABC superfamily.

Figure S2 (.aln format) Multiple alignment of recognizable 8 and 10 TMS integral membrane protein homologues of the ABC superfamily.

Figure S3 (.aln format) Multiple alignment of N- and C-terminal repeat segments of 8 and 10 TMS integral membrane protein homologues of the ABC superfamily.

Figure S4 (.doc format) Binary alignment of the N- and C-terminal 4 TMS repeat units of Sav4. The predicted TMSs are shaded and labeled by number.

Figures S5(A)-S7(A) (.doc format) PAUP trees without bootstrap values for the 4 TMS, 8 and 10 TMS, and the N- and C-terminal halves of the 8 and 10 TMS integral membrane protein homologues of the ABC superfamily.

Figure S5(B) (.log format) PAUP tree with bootstrap values for the 4 TMS proteins.

Figure S5(C) (.doc format) Clustal X tree for the 4 TMS proteins.

Figure S6(B) (.log format) PAUP tree with bootstrap values for the 8 and 10 TMS proteins.

Figure S6(C) (.doc format) Clustal X tree for the 8 and 10 TMS proteins.

Figure S7(B) (.log format) PAUP tree with bootstrap values for the N- and C-terminal halves of the 8 and 10 TMS proteins.

Figure S7(C) (.doc format) Clustal X tree for the N- and C-terminal halves of the 8 and 10 TMS proteins.


Tables S1-S4 (.doc format) Analyses of the integral membrane protein homologues of the ABC superfamily.