Figure S1B’

1

Mba1 ---------------------------------FGAQGGSKP------------------

Psp2 ---------------------------------LSTGVTNEV------------------

Ybe1 --------------------------------WLNNFGTARLQANVDDRG----------

Ype5 --------------------------------WFNQFGSAKVQLNLDEKL----------

Eco14 --------------------------------WLNQFGTAKTQLSVVSDF----------

Sen3 --------------------------------WLQQFGTAKVNLGLDKDL----------

Ymo1 --------------------------------WLGHIGKAQVKLQVDDKN----------

Yen2 --------------------------------WLGHIGQAQVKLQTDDKN----------

Pas1 -------------------NDDVMQGSDISKSGIADMGFAALQPETEKSAGEVRANLPLS

Yfr1 --------------------------------WLGQYGNARVQLNSNS------------

Yfr5 --------------------------------WLSQFGHARVQLNSSG------------

Yps7 --------------------------------WLSQFGTARVQLNINDDF----------

Yfr2 --------------------------------WLSQFGTARVQMNVNDDF----------

Yfr3 --------------------------------WLSQFGTARVQLNLDNNL----------

Yps4 --------------------------------WLNQFGTARVQLNVDSDF----------

Yps2 --------------------------------WLNRFGTAQVNLNFDKNF----------

Esa2 --------------------------------WLSHFGTAQVTLDVDDNG----------

Plu1 --------------------------------WLSQFGTAKINLNVDHRG----------

Eco1 --------------------------------WLQHYGTAEVNLQSGNN-----------

Pru1 --------------------------------WFNRFGSSQIHLEADKKF----------

Pal3 --------------------------------WFNHFGSSQIKLEADKKF----------

Eta1 --------------------------------WLNQFGTARVQLDADEHF----------

Sgl1 --------------------------------WLSQFGTARLQLDVDNKF----------

Eco26 --------------------------------WFENFGTAHIQLQVDKNF----------

Pmi1 --------------------------------WLNQFGHARVSLSADKNL----------

Efe5 --------------------------------WLQQYGNARIKLNVDDSF----------

Eco20 --------------------------------WLGKYGTARVKLNVDKDF----------

Eco25 --------------------------------WLSRFGTARITLGVDEDF----------

Eco15 --------------------------------WLNNFGTAXISLGVDEDF----------

Eco6 --------------------------------WLSRWGTARVSLGVDEDF----------

Efe2 --------------------------------WLSQWGTARVTLSADEHF----------

Efe3 --------------------------------WLSQWGTVRVTLGLDEDF----------

Pal2 --------------------------------WLSKAGNVKLNIDFDKKF----------

Csu1 ---------------------------------------LRFDFFPGDRE----------

Eco3 --------------------------------WLSPWGNASVDVKVDNEG----------

Cko1 --------------------------------WLSPWGNASVDLRIDNEG----------

Sen1 --------------------------------WLSAWGSASVDVKVDNEG----------

Eca1 --------------------------------WLSPWGNASVNVRVDDNG----------

Esp2 --------------------------------WLSPWGNASVDLLVDEEG----------

Esa3 --------------------------------LLSPWGNATVDLLVDEEG----------

Kpn1 --------------------------------WLSPWGNANVDLLVDKEG----------

Pan1 --------------------------------LLSPYGNAEFNLNVDMAG----------

Eta2 --------------------------------LLSPYGRASVSLALSDDG----------

Spr1 --------------------------------LLSPLGNAKLSLVMSDQG----------

Yin1 --------------------------------LLGKFGKAQVNLAVDDKG----------

Yin2 --------------------------------LLGKFGKAQVNLAVDDNG----------

Yfr4 --------------------------------LLGKFGQAQVSIAVDDHG----------

Ymo2 --------------------------------LLGRFGQAQVNLSMDNKG----------

Eco10 --------------------------------TLSPYGKVRSNLSIGQGG----------

Eal1 --------------------------------ALSPYGKVRSNLSIGQGG----------

Sty4 ---------------------------------MSPYGKIRTSLSVGEGG----------

Eco16 --------------------------------VLSPYGSVRTSLSIGEGG----------

Ahy1 SASRYGSEQEVQYWRQQLATQFEEEANAYAASLLGAMGTARTRVTLDDDF----------

Sen2 -------------------------------------IIFRGGVNLEDGA----------

Efe4 -------------------------------------AQFRGGITLEDAS----------

Bpe1 --------------------------------GLPFLRNLQGGLSHDFES----------

Bav2 --------------------------------GLRFLRNLQGDLRHDFDN----------

Bbr1 --------------------------------ELPFLRRLQGGVNYDFSN----------

Bpa2 --------------------------------GLPWLRRLEGNVSYDFSG----------

Bav1 --------------------------------GLPWLRHLDGGLRYDLDP----------

Pma1 ---------------------------------AQTSVNISGGTESDTSF----------

Pma3 ---------------------------------AQTNFNISGGSESDTSF----------

Pma5 ---------------------------------AQTSLNIGGGTESDTSF----------

Pma4 ---------------------------------AQTTVSINGTSNSETSF----------

Ssp1 ---------------------------------AQTTLGLDAATSSDLTG----------

Ssp2 ---------------------------------SQTEFGVGFESDADMTY----------

Plu2 ----------------------------------TNAPS---------------------

Cla1 ---------SKSKEEIVKNMASSYLNTSANELAKEFIDSLNTSINTDFSFN-------YN

Rba1 ----------------------------------LSVGSDTMGLDKNKSD----------

2 3 4

Mba1 -----SGGLLVVAPLSDPDDIFNTYFTQGSVFY----EDN-RTTLNLGLGYRK------L

Psp2 -----TGSVLVVKPISDPSDNENIIFTQASLFL----SDDSRETINLGFGNRK------L

Ybe1 --DLDGSQFDMLMPFYDTPS--QMAFTQFGIRR-----IDKRTTANLGIGIR-------H

Ype5 --SLKGSQLDVLLPLTDSPD--LLTFTQLGGRY-----IDDRVTLNVGLGQR-------H

Eco14 --SLKGSSLDVLLPFYNTPK--NVLFTQLGMRD-----NDGRFTTNAGLGHR-------Y

Sen3 --SLDNASLDLLLPLYDDKK-QNLLFTQWGGRR-----DDDRNIINVGMGYR-------Y

Ymo1 --DFSGSELHLFVPLYNQPE--RLAFSQFGFRR-----IDQRNIMNIGLGQR-------H

Yen2 --DFSGSEIDLFIPLYDQPE--KLAFSQFGFRR-----IDQRNIMNIGLGQR-------H

Pas1 DGKLTSGSIDLFYPLYDGDS--RLFFGQVGARR-----FDGRNIVNLGIGQR-------Y

Yfr1 -----IGNADVLIPLTETQ--NNLLFGQLGVRY-----NGERTTNNVGLGVR-------S

Yfr5 -----TGNADILLPLVESQ--DNLLFGQLGVRY-----DGYRTTNNAGIGVR-------Q

Yps7 --HLDGSAADVLIPLYDNE--KSILFTQLGARN-----KDSRNTVNMGAGVR-------T

Yfr2 --KLDGSAVDVLVPIYDNQ--KSILFTQLGARN-----KDNRNTVNIGAGVR-------T

Yfr3 --SLKGSAFDMLLPLYDDQ--KSLLFSQFGLRN-----HDSRNTINIGAGVR-------T

Yps4 --KLDNSALDLLVPLKDSE--SSLLFTQLGVRN-----KDSRNTVNIGAGIR-------Q

Yps2 --SLKESSLDWLAPWYDSA--SFLFFSQLGIRN-----KDSRNTLNLGVGIR-------T

Esa2 --NWDNSAFDFLAPLYDNK--KSVLFTQLGIRA-----PDGRTTGNIGLGVRT------F

Plu1 --RLDESSVDLLVPFYDDKD-HWLVYSQYGYRH-----KDSRDTVNLGIGTR-------L

Eco1 ---FDGSSLDFLLPFYDSE--KMLAFGQVGARY-----IDSRFTANLGAGQR-------F

Pru1 --SLKNSQIDLLMPWYETE--DNLIFSQTSLHR-----KEGRIETNLGLGAR-------W

Pal3 --SLKNSQIDLLLPLYETE--NNLLFTQTSLHR-----KEGRIETNLGLGTR-------W

Eta1 --SFKNSQFDLLAPLYEQK--DSLIFTQGSLHR-----TDDRTQVNLGFGLR-------Y

Sgl1 --SLKNSQLDLLIPLYEQP--DKLVFTQGSLHR-----TDDRTQTNLGMGMR-------W

Eco26 --SLKNSQLELLFPVFEDD--ERLFFSQGGISY-----IDDKFISNIGIGYR-------A

Pmi1 --TLKNSSAELLIPLYEQK--EKLIFAQTNYHR-----KDLRSQFNYGIGYR-------Y

Efe5 --SLKDAEFDFLYPWMDTK--DYVLFSQTSLHR-----TDDRNQANIGIGVR-------H

Eco20 --SLKDSSLEMLYPIYDTP--TNMLFTQGAIHR-----TDDRTQSNIGFGWR-------H

Eco25 --SLKNSQFDFLHPWYETP--DNLFFSQHTLHR-----TDERTQINNGLGWR-------H

Eco15 --SLKNSQFDFLHPWYDTP--DYLLFSQHTLHR-----TDDRTQINTGLGWR-------H

Eco6 --SLKSSSFEFLHPWYETP--DNLVFSQHTLHR-----TDDRTQTNHGIGWR-------Y

Efe2 --TLKGSALDLLLPWYDTP--ENIIFTQHSIHR-----TDDRNQLNTGTGWR-------H

Efe3 --TLKGSAFDLLLPWHDTP--ENLLFTQHSFHR-----TDDRNQLNTGAGWR-------H

Pal2 --SIKNSQFDWLIPWYDQE--DILLFTQHTLHR-----YDERFHTNNGIGLR-------Y

Csu1 -YPFERTAIDWLFPFYDSL--EFLFFSQLGFRHN----LDRRDIINLGGGGR--------

Eco3 --HFTGSRGSWFVPLQDND--RYLTWSQLGLTQ-----QDDGLVSNVGVGQRW------A

Cko1 --SFTGSRGGWFIPWQDNT--RYLTWSQLGLTQ-----QDDGLVSNVGIGQRW------A

Sen1 --HFNGSRGSWFIPLQDKQ--RYLTWSLLGLTQ-----QTDGLVSNIGVGQRW------V

Eca1 --TFNGSSGSWFIPWNDNN--RYLSWSQLGLTQ-----QTDGLVSNAGVGQRW------V

Esp2 --KFNGSSGRWFIPWQDNN--RYLSWSQLGLTQ-----QTDGLVSNAGIGQRW------V

Esa3 --NFNGSSGSLFTPWQDNN--RYLTWSQVGVSQ-----QNQGLVGNAGIGQRW------A

Kpn1 --KFTGSKGSWFVPLQDND--RYLTWNQYSVTR-----REHDLVGNIGLGQRW------R

Pan1 --NFDGTGGNLFSPLADEN--RYLTFSQLGLHD-----SVEGVVGNAGLGQRW------D

Eta2 --SFNGTSAQLLTPWQDNY--QYLTFSQLGIEQ-----SEYGTVGNAGLGQRW------I

Spr1 --DFSGSSGQLFSPLYDVN--GLLTYSQLGLLQ-----QTEGSLGNFGLGQRW------V

Yin1 --SLSKSSFSLFSPWYEND--AMVAFSQVGIHG-----QDGRMIGNLGAGVRF------D

Yin2 --SLSKSAFSLFSPWYEND--AMVAFSQVGVHR-----QDNRMIGNLGAGVRF------D

Yfr4 --DFSKSTFSLFTPWYEND--AMVAFSQVGIHD-----QDSRTIGNFGAGLRW------D

Ymo2 --NLNRSTASLFTPWYDSE--QYLLFSQINIHH-----QDNRKIGNFGLGHRI------E

Eco10 --DLDGSSIDYFVPWYDNQ--TTVYFSQFSAQR-----KEDRTIGNIGLGVRY------N

Eal1 --DLEGSSIDYFIPWYDDQ--STVYFSQFSAQR-----KEERTIGNAGIGVRH------N

Sty4 --DLDGSSLDYFVPWYDNE--KTLLFSQLSAQR-----KEDRTIGNFGLGVRQ------N

Eco16 --DLDGSTFDYFVPWYDNQ--ETLVFSQFSAQR-----KDDRTIGNIGVGVRQ------N

Ahy1 --NMVTAEADLLLPLAEEQ--QTLLFTQFGLRRN----GQDRTIANLGVGQRH------F

Sen2 --KYRSSEFDMFIPVQETT--SSLLFGQLGFRDHDSSSFDGRTFANVGMGYRQ------E

Efe4 --KYRSAEADLLIPLYQST--SSILFGQLGLRDHDNNSFNGRFFVNTGIGWRQ------D

Bpe1 --GRTSLQLNTIDEVYRAG--RNTGLLQLGAHN-----QNDRPTANAGAVYR-------R

Bav2 --GRTSLELRTIDQVYRKG--ANTGLLQLGGHN-----QNNRPTANLGGVYR-------R

Bbr1 --KDLSLDLRTIDEVHRGE--RDRVLLQLSGHN-----RNHRPTVNGGVVLR-------H

Bpa2 --RDVAVDVRTIDALHLDQ--DRALLLQLGGHN-----QNHRPTVNAGVVAR-------S

Bav1 --GRLSFSLRTIDDLMVSE--RRALMLQAGLHN-----QNQRPTANTGIVLR-------Q

Pma1 --SINSLMKLGELAKDDQGDLKTLAFSQARFATAT---NAEGSTINIGLGIR-------N

Pma3 --SINSLMKLGELAKDEEGDLKTLAFSQARFATAT---NADGSTFNLGLGIR-------N

Pma5 --SLNSLMKLGELAKDDEGDIKTLAFSQARLATAT---NAEGSTTNLGLGIR-------H

Pma4 --SLDSLMKLREMATDDEGDIKTLLFSQAKLSKTS---NSDGTTANLGLGIR-------H

Ssp1 --YLDSFMRLKTIGYDNEGDPMGLMFGQARVTLET---SAQ-PQVNVGLGSR-------F

Ssp2 --YLNSLISLAQLGSDDNGYPLGLLFAQG-SAKGA---YSGSAVTNLGLGLR-------R

Plu2 --WVTSWDVTVPCPVYVSENQADNIFFEGGFDY-----QDARKTVDGALGYRH------L

Cla1 ERSGFSGNAKALLPIVSEDNPKISYFLQSGIGEF----ANDRTIGHFGGGIRYYPNATAL

Rba1 ----TKTEAMTVYRLKETG--NWFLFNQTSAVN-----FNNRTTINTGFGARH------I

. . *

5 6 7

Mba1 SDNKMLLTGINAFYDHEFP---YDHGRTSIGLEARTTVWEINANKYWATTKWKTGK----

Psp2 INDDTLLVGYNLFYDHELD---YDHQRASIGIEAISSVGSLRANQYYGLSGWKSGL----

Ybe1 FIDD-WMVGYNLFLDRDIT---RDHTRVGAGAEYARDYLKLAANGYLRLSDWRDS--PDF

Ype5 FFAQ-QMLGYNLFIDHDAS---YSHTRIGVGAEYGRDFINLAANGYFGVSGWKNS--PDL

Eco14 FTDNGWMLGYNVFYDVDWR---NTNRRYGIGVEAWRDYLKLSANGYKRLSDWRQS--PTV

Sen3 FADR-WMWGINTFYDRQISD--NAHERLGIGGELGWNYFKLSANGYKRLSGWKDS--SEY

Ymo1 YLSD-WMLGYNVFLDQQISG--NAHRRLGLGGELARDYVKLSANSYYRLGGWKNS--TRL

Yen2 YVSD-WMFGYNIFFDQQVSG--NAHRRVGFGGELARDYIKLSANSYHRLGGWKNS--TRL

Pas1 FQGD-WALGYNTFYDIQISG--NAHQRLGFGLEYWRDYLYLSANGYFGLTDWYSS--SAL

Yfr1 FTDS-WMFGVNTFYDYDLT---GKNSRLGVGGEAWTDNLKFSANGYFRLTDWHQSVLADM

Yfr5 FTEN-WMFGVNSFYDYDLT---GKNSRVGVGAEAWTDNLKFSANGYFRLTDWHQSVLSDM

Yps7 FQGN-WMYGANTFFDNDLT---GKNRRIGVGAEAWTDYLKLSANNYFGITDWHQS--RDF

Yfr2 FQNN-WMYGVNTFFDNDMT---GKNRRVGVGAEAWTDYLKLSANSYIGTSDWHQS--RDF

Yfr3 LQDN-WMYGANVFFDRDIT---GKNNRIGFGAEAWTDYLKLSANSYLRLTDWHQS--RDF

Yps4 YQGD-WMYGANTFFDNDLT---GKNRRVGVGAEVATDYLKFSANTYFGLTGWHQS--RDF

Yps2 LENG-WLYGLNTFYDNDLT---GHNHRIGLGAEAWTDYLQLAANGYFRLNGWHSS--RDF

Esa2 YVRD-WMFGGNVFFDDDFT---GENRRIGFGAEAWTNYLKLSANTYIGTSQWHNS--GDF

Plu1 FINN-WMYGANTFYDNDLT---GNNSRFSLGGELWTNYLKMSANAYFRLSDWHNA--RDL

Eco1 FLPE-NMLGYNVFIDQDFS---GDNTRLGIGGEYWRDYFKSSVNGYFRMSGWHES--YNK

Pru1 YGE-GQMIGGNTFFDYDIS---RKHSRLGLGVEYRRDFLKLSANSYHRLSGWRSS--RDL

Pal3 YGE-NQMIGGNSFFDYDIS---RKHSRLGLGVEYRRDFLKLSANSYHRLSGWRNS--RDL

Eta1 FAP-SYMLGGNIFGDYDLS---RAHSRTGIGMEYWRDFLKLSANGYLRLSDWNNS--SDF

Sgl1 FND-GYMLGGNTFLDYDLS---RDHARMGMGVEYWRDYLKIGANNYLRLTNWRDS--KDF

Eco26 FYD-NWMLGGNSFIDYDLR---KEHSRLGLGIEYWQDNLKLGANSYLRLSNWRNS--SNI

Pmi1 FTE-KFMVGINGFYDHDLT---HHHNRLGIGAEIWRDYFKLSSNHYHRLSSWRAS--NNI

Efe5 FTPDNAMLGANIFYDYDLT---RSHSRAGFGVEYWRDYLRFGANTYFGLSDWKNS--RDL

Eco20 FSGNDWMAGVNTFIDHDLS---RSHTRIGVGAEYWRDYLKLSANGYIRASGWKKS--PDI

Eco25 FTP-TWMSGINFFFDHDLS---RYHSRAGIGAEYWRDYLKLSSNGYLRLTNWRSA--PEL

Eco15 FTS-SWMSGINLFFDHDLS---RYHSRAGLGAEYWRDYLKLSSNAYIGLTGWRSA--PEL

Eco6 FTS-SWMSGVNMFIDHDLT---RYHTRTGMGVEYWRDYLKLSGNGYLRLSNWRSA--PEL

Efe2 FMP-DYMTGVNLFFDHDLT---RYHSRMGLGGEYWRDNLKLGANGYLRLTGWRDA--PEL

Efe3 FAP-DYMAGVNLFFDHDLT---RYHSRMGLGGEYWRDNLKLGANGYLRLSGWRDA--PEL

Pal2 FHE-KSTIGMNAFIDHDLS---HAHTRVGLGVEYWQDYLKLNANSYFGLTSWKSA--SEL

Csu1 FFRKNWMLGANAFCDCDLT---GTNTRLGLGLESWTNFLQFSMNGYLGLSSPRHS---SL

Eco3 RG--NWLVGYNTFYDNLLD---ENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQ----T

Cko1 RD--GWLLGYNTFYDNLLD---ENLQRAGFGAEAWGEYLRLSANYYQPFASWHEQ----T

Sen1 QN--GWLLGYNTFYDNLLD---ENLQRAGVGAEAWGEYLRLSANYYQPFADWQTH----T

Eca1 AG--NWLLGYNTFYDNLLD---ENLQRAGLGAEAWGQNLRLSANYYQPFAGWRDD----S

Esp2 AG--KWLLGYNTFYDNLLD---ENLQRAGLGAEAWGENLRLSANYYQPFASWRDS----S

Esa3 AG--HWLLGYNTFYDRLFD---DDTSRAGFGAEAWGDYLRLSANYYQPLGGWQHR----A

Kpn1 VG--GWLLGYNSFYDKVLS---ESLARGSVGAEAWGEYLRLSANYYHPLGDWQLRD---N

Pan1 AG--NWLLGYNSFIDRSFR---TGLQRASVGTEAWSNNLRFSANYYHPLSGWRNRG---D

Eta2 AG--SWRVGYNAFVDGLLG---SDRQRGSLGAEAWGEYLRLSANYYHPLSGWRNRS---N

Spr1 AG--DWLLGYNTVLDSDFE---RHHNRASLGAEAWGDFLRFSANYYYPLSALAQQR---D

Yin1 QG--DWLLGANTFFDQDIS---RNHSRLGLGLEWWADNLKLATNYYHPLSGWKDSK---D

Yin2 QG--DWLFGANTFLDQDIS---RNHSRLGLGLEWWADNLKLASNYYHPLSGWKDSK---D

Yfr4 KG--NWLLGYNTFLDQDFS---RNHSRLGLGAELWTESLRLATNFYHPLSGWKDSK---D

Ymo2 LPSLNGLLGYNVFIDHDFS---RGHNRAGIGAEARADYLKFSANYYHPLSHWKDSP---D

Eco10 FD--KYLLGGNIFYDYDFT---RGHRRLGLGAEAWTDYLKFSGNYYHPLSDWKDSE---D

Eal1 FD--KWLLGGNIFYDYDFT---RGHRRLGLGTEAWTDYLKFSGNYYHPLSGWKNSE---D

Sty4 VG--NWLLGGNAFYDYDFT---RGHRRLGLGTEAWTDYLKFSGNYYHPLSDWKDSE---D

Eco16 IG--SWLLGGNLFYDYDFT---RGHRRLGLGSEAWTDYLKFSGNYYHPLSDWKDSK---D

Ahy1 LDR--WMLGYNLFADYDLT---NRHWRAGVGAEAWRDYLKLGANFYTPLSSWRDSP---R

Sen2 VNG--WLLGVNTFLDADIR---YSHLRGGIGGEVYKESLAFSGNYYFPLTGWRTS---AA

Efe4 VGD--WLLGINSFLDADVR---YDHLRGSLGVELFRDSMSLAGNWYFPLSDWKAS---KV

Bpe1 EVNDALMVGANGFLDYEFG---KQHLRGSVGLEVIAPEFSLYGNVYAPLSDWKGAK---R

Bav2 DINERLMLGANAFLDYEFA---KQHLRGSLGVEAIAPEFSFYGNVYAPMSGWTGAK---R

Bbr1 ALNQHMAVGANAFLDYEFG---KNHLRGSLGGEVIAPQFTLYGNVYAPMSGWKAAK---R

Bpa2 AAGSSLILGGNAFLDYEVG---KRHLRGSLGAEAVAAQFTLYGNVYAPLSGWKAAK---R

Bav1 QASPGLIVGSNAFLDYEFG---KQHVRGSLGLEAIAPHYSLYANYYAPLSGWKGAR---R

Pma1 RPDDISMVGANAFWDYRMTDYSDAHSRLGLGGEYFWKDFEFRNNWYMAITNEKDVIIKGV

Pma3 RPNDISMVGANAFWDYRMTDYSDAHSRLGLGGEYFWKDFEFRNNWYISITDEKDVTIKGV

Pma5 RPNDVSMLGANAFWDYRMTDYSDAHSRLGLGGEYLWKDFELRNNWYMSITNEKDVTIKGV

Pma4 RPNNESMMGANAFLDYRMTDYSDSHSRLGLGGEYLKNGWELRNNWYMSMTEQKNVTVNGT

Ssp1 RLGDEAIVGLNGFWDLRTTNYSTAYTRWGIGAEGFWKSFELRNNWYINGSADKNITINNI

Ssp2 RLRDNAMLGANAFWDYRFTNYSSSYSRWGAGAELWWDDFKLTNNWYIAGTGIKRITTSGR

Plu2 MSDNKVMLGANVLYSHEFP---RNHQRISYGAEIRTSVFEINSNYYHRLTDWKLTG---V

Cla1 NNSGNIMLGLNSVYDHDFS---RGHKRMSLGAEAMVDTLAFNANVYQRLSSWIDSY--DF

Rba1 NDANTVITGYNIFYDYELQ---SKHERVGAGLELLSSIFEFRANAYQAVSKTLTYN----

* * . . * . * * * *

8 9

Mba1 -NGLE--------------------ERALDGYDIEAGVPLPYMNWATVFVKNFQWDSEIS

Psp2 -NNIN--------------------EKALNGSDVELGMPLPYLPWTNLYYRSFNWEG-AS

Ybe1 S-SYS--------------------ERPATGFDLRAEAYLPSLPQLGGKLMYEQYFGNDV

Ype5 D-KYD--------------------EKVANGFDLRSEAYLPTLPQLGGKLIYEQYFGDEV

Eco14 T-DYD--------------------ERPADGWDIRAEGWLPAYPQLGGKLVYEQYYGNEV

Sen3 E-DYQ--------------------ERVANGYDIRAEGYLPAWPQLGAQLVWEQYYGDDV

Ymo1 E-DYD--------------------ERAASGYDIRTEAYLPYYPQLGGKLMYEQYFGNEV

Yen2 E-DYD--------------------ERAANGYDIRTEAYLPHYPQLGGKLMYEQYFGDEV

Pas1 D-GYA--------------------ERAANGYDIRAQGWFPVYPQLSGKLKFEQYFGDDI

Yfr1 E-DYN--------------------ERPANGFDVRAEAYLPSYPQLGGRLMYEKYFGKGV

Yfr5 E-DYN--------------------ERPANGFDVRAAAYLPAYPQLGGSLMYEKYFGKGV

Yps7 I-DYN--------------------ERPANGYDLRAEAYLPSYPQLGGKAMYEKYRGDDV

Yfr2 A-DYN--------------------ERPANGYDVRAEAYLPSHPQLGGKLMYEKYRGEEV

Yfr3 A-DYN--------------------ERPANGYDLRVEAYLPAYPQIGTNLKYEQYKGNEV

Yps4 S-SYD--------------------ERPADGFDIRTEAYLPAYPQLGGKLMYEKYRGDEV

Yps2 S-DYK--------------------ERPATGGDLRANAYLPALPQLGGKLMYEQYTGERV

Esa2 D-NYN--------------------EKPADGYDVRAEGYLPSFPQLGAKLMYEQYYGDNV

Plu1 V-NYY--------------------ERPANGYDLIADMYLPSMPSLGAKIKYEQYFGDNV

Eco1 K-DYD--------------------ERPANGFDIRFNGYLPSYPALGARLMYEQYYGDNV

Pru1 A-DHS--------------------ARPSNGWDVRAEGWLPSYPHIGGKLTYEQYYGDSV

Pal3 A-DYS--------------------TRPANGWDLRAEGWLPIYPHIGGKLTYEQFYGEEA

Eta1 K-DYQ--------------------ERPANGWDIRAQAWLPSLPQLGGKLTYEQYYGRGV

Sgl1 A-DYQ--------------------ERPANGWDMSLEGWVPALPQLGGNLKYEQYYGKEV

Eco26 V-DYE--------------------ERPANGLDLNIKSWLPSYPQIGGDIKYEKYYGDDV

Pmi1 L-DYS--------------------ERPANGWDIRTEGYFPAYPQLGTKLIFEQYYGKEV

Efe5 A-DYL--------------------ERPANGWDVSAEGWLPAYPQLGASVQFEKYYGKNV

Eco20 E-DYQ--------------------ERPANGWDIRAEGYLPAWPQLGASLMYEQYYGDEV

Eco25 DNDYE--------------------ARPANGWDVRAEGWLPAWPHLGGKLVYEQYYGDEV

Eco15 DNDFE--------------------ARPANGWDLRAEGWLPAWPQLGGKLVYEQYYGDEV

Eco6 DNDYE--------------------ARPANGWDLRAEGWLPAWPQLGGKLVYEQYYGDEV

Efe2 DYDYE--------------------ARPANGWDVRAEGYLPAYPQLGAKLMYEQYYGDEV

Efe3 DYDYE--------------------ARPANGWDVRAEGYLPAYPQLGATLMYEQYYGDEV

Pal2 NHDFN--------------------AKPAHGWDIQVEGWLPNYPHLGGNLRYEQYYGDSV

Csu1 QPNYQ--------------------ESPAHGLDFRLHYWLPFFPRLGGKIFYEQYFGKQV

Eco3 ATQE---------------------QRMARGYDLTARMRMPFYQHLNTSVSVEQYFGDRV

Cko1 TALE---------------------QRMARGYDVTAQMRMPFYQHLNTSVSVEQYFGERV

Sen1 ATLE---------------------QRMARGYDINAQMRLPFYQHINTSVSLEQYFGDSV

Eca1 DVQE---------------------QRMARGYDVTAKAWLPWFHHLNTSVSFEQYFGDSV

Esp2 DLQE---------------------QRMARGYDVTAKAWLPYFHHLNTSVSFEQYFGDNV

Esa3 GLLE---------------------QRMARGYDVTAQAYLPFYQHINTSVSFEQYFGDQV

Kpn1 QTQE---------------------QRMAAGYDVTAQARLPFYQHINTSVSVEQYFGDSV

Pan1 SQQ----------------------SRMAEGYDITTQSYLPFYRQLGVSFSYQQYLGEKV

Eta2 SSQ----------------------MRMARGYDITTRGYLPFYHQLGVTLSYEQYLGDRI

Spr1 NAQFL--------------------SRPASGYDITTQGYLPFYRQIGGSLSYEQYWGENV

Yin1 FDDYL--------------------ERPARGFDVRAQGYLPAYQQLGASVVYEQYYGDEV

Yin2 FDDYL--------------------ERPARGFDVHAQGYLPAYQQLGASAVYEQYYGDEV

Yfr4 FDDYL--------------------ERPAKGFDVRLQGYLPAYPHLGASVVYEQYYGDEV

Ymo2 FDDYL--------------------ERPAKGYDLRSQGYLPAYPQLGVSAVYEHYFGDEV

Eco10 FDFYE--------------------ERPARGWDIRAEAWLPAYPQLGGKIVFEQYYGNEV

Eal1 FDFYE--------------------ERPARGWDIRAEAWLPAYPQLGGKIVFEQYYGDEV

Sty4 FDFYE--------------------ERPARGWDIRMESWLPFYPQLGAKLVYEQYYGDEV

Eco16 FDFYE--------------------ERPARGWDVRAEGWLPAWPQFGAKLVYEQYYGDEV

Ahy1 FEGME--------------------ERAARGMDVRLEAYLPAYPQWSASLTAEQYLGERV

Sen2 HEFHD--------------------ERPAYGFDLRTKGTLPDFPWFSGELTYEQYYGDKV

Efe4 QPLHD--------------------ERPATGIDVRLKGALPSLPWFGAELAFEQYFGDKV

Bpe1 NNRRE--------------------EKPASGMDVGVGYRPAFAPGLSLSATHFRWNGAEV

Bav2 DNRRE--------------------ERPASGMDLGMKYSPGFAPGLSLKANYFRWNGAAV

Bbr1 AERRE--------------------ERPASGWDVGVRLQPEALPGLAIKGQYFRWSGAAV

Bpa2 AERRE--------------------ERPAAGWDVGFTARPEAVQGLALNAQYFRWRGAQV

Bav1 DSRRE--------------------ERPAAGYDLGGQLS--SDAGLSLQAAYFRWHGAGI

Pma1 DYQ----------------------ERVVPGWDLEVGYRLPNNPELAFYIRGFNWDYKYT

Pma3 SYK----------------------ERVVPGWDVELGYRLPNNPELAFFVRGFNWDYKHT

Pma5 SYK----------------------ERVVPGWDVELGYRLPNNPELAFFVRGFNWDYKNT

Pma4 VYT----------------------ERVVPGWDVEAGYRFPNHPEMAVFVKGFNWDYKNT

Ssp1 DYV----------------------ERVVPGWDVEVGYRIPSYPQLAIFVRGFNWDYQDH

Ssp2 AYTDTTSLAAGTYDETTLLGANTFDERVVPGWDVALNYRLPSYPQLSLGIRGFRWDYMRK

Plu2 DNNE---------------------EKARGGYDVELALAVPYVPSAHFRVKHFCWNGIAS

Cla1 DKDYVQ-------------------ERPANGWDAKIKYAFPSLINVSFFAKMGQWYGNKV

Rba1 --GIQ--------------------ETALDGYDAKLTANLPYFYSS-------NLYGKLS

* *

10 11 12

Mba1 GSKD---------IKGNDLQLRAYIPGITGLEIQAGRTFFSDSSGTD--ENYINIFYNVT

Psp2 GAAD---------LEGDEISLEAKLTNFN-IEIGK-RS--NDGVTED--EEFLKITYTCC

Ybe1 GLFG--KDN--RQQNPAAITAGINYTPIPLVTVGIDRKQGSAGNGET--LFNLGVNYEVG

Ype5 GLFG--VDN--RQKNPLAVTLGVNYTPIPLFTVGVDHKMGRAGMNDT--RFNLGFNYAFG

Eco14 ALFG--ESE--RQKNPHAITAGVTWTPFSLLTAGVDYRRGKNGADDT--RLNLGLTYRIG

Sen3 ALFD--DSEDDRQRNPYAVTAGVNYTPFPLVSIGLNQKMGKGNHNDT--QIDLAVNWMLG

Ymo1 ALFG--LNE--RQKNPSALTASVSYTPFPLVNLALEHTIGNSGKNKT--GVNLAVNYEIN

Yen2 ALFG--INE--RQKNPSALTAGVSYTPIPLVSLGLDHTIGNGGKKKT--GVNVAVNYEIN

Pas1 ALLN--HQN--RYKNPYALTMGLEYTPIQLISLGIDRTFSHRGKDDT--KVNLSFNYQLG

Yfr1 ALNSGSTSPDDLGDSPSAFTVGLNYTPIPLFTVDVAHKKGQNTNNEL--QLGLNFNYRFG

Yfr5 ALNSGSTSPDDLGDSPSAVTLGVNYTPIPLITVDVAHKKGQNTSNEL--QVGLNFNYRFG

Yps7 ALFG----KDNRQKNPHAITAGVNYTPIPLVTIGAEHRAGKGGQNDS--NINFQLNYRLG

Yfr2 ALFG----KDNRQKNPHAVTAGVNYTPIPLLTVGAEHRAGKGSKNDS--SINFQFNYRLG

Yfr3 ALFG----KDDRQKNPYAFTAGINYTPIPLITIGAEQRAGKGGRNDT--NISIQLNYRLG

Yps4 ALFG----KDDRQKDPHAVTLGVNYTPVPLVTIGAEHREGKGNNNNT--SVNVQLNYRMG

Yps2 ALFG----KDNLQRNPYAVTAGINYTPVPLLTVGVDQRMGKSSKHET--QWNLQMNYRLG

Esa2 ALFD----KDHLQSNPSAVTVGLNYTPVPLITAGIDYKRGQDSMDEM--KFSLNFHYALD

Plu1 ALFG----KNKRQKDPYAATIGVNYTPIPLITAGIDYKLGKEGKSDG--IFSFNVNYRFG

Eco1 ALFN----SDKLQSNPGAATVGVNYTPIPLVTMGIDYRHGTGNENDL--LYSMQFRYQFD

Pru1 ALFG----TKNLQQNPYSITAGLNYTPIPLVTFNAEHRQGKASKQDS--RFGLQLNYQFG

Pal3 ALFG----TKNRQNNPYSITAGINYTPIPLITLNGEHRQGKASKQDS--RIGLKLSYQFG

Eta1 ALFG----KENLQQDPRAITAGVNFTPFPLLTLNAEHRQGASGKNDK--RLGVDFSYQLG

Sgl1 ALFG----KDNRQKDPHAITVGVNYTPFPLLTFSADQRQGKAGQNDT--RLGVQLNIQLG

Eco26 ALFG----ENHRQRNPHSTTLGISYTPFPLMSFKAEHKMGSNNINDS--RIGFEINYQIH

Pmi1 GLFG----KDKRDKNPHTYTLGINYTPIPLVTLNAERRIGLHDRADN--NLNINLSYRIG

Efe5 GLFG----SDNLQENPYAVTGEISYTPVPLVKFSAQHRQGQSNTHDT--RFGVEINYRPG

Eco20 GLFG----KDKRQKDPHAISAEVTYTPVPLLTLSAGHKQGKSGENDT--RFGLEVNYRIG

Eco25 ALFD----KDDRQSNPHAITAGLNYTPFPLMTFSAEQRQGKQGENDT--RFAVDFTWQPG

Eco15 ALFD----KNDRQSNPHAITAGLNYTPFPLLTLSAEQRQGKQGENDT--RFAVDLTWQPS

Eco6 ALFG----KDERQNDPHAITAGLSYTPVPLISFSAEQRQGKQGENDT--RIGMELTLQPG

Efe2 ALFG----RDHRQKDPHAFTAGVSYTPVPLVSLSAEQRQGKGGENDT--RFGLNLSYTPG

Efe3 ALFG----KDKRQQDPHAFTAGLSYTPVPLISLSAEQKQGKGGENDT--RFALNLTYTPG

Pal2 ALFG----KTKRQKNPNAATIGANWTPFPLFTLNASHKLGSEKQVET--QAKLQFTWTFG

Csu1 SLFG----RSDLQHDPFVITTGVEFTPFPLLSMEIDHRMSKASRHYT--LFNIRLNYRFG

Eco3 DLFN----SGTGYHNPVALSLGLNYTPVPLVTVTAQHKQGESGENQN--NLGLNLNYRFG

Cko1 DLFD----SGTGYHNPVAVKLGLNYTPVPLVTVTAQHKQGESGVSQN--NLGLNLNYRFG

Sen1 DLFD----SGTGYHNPVALKLGLNYTPVPLLTMTARHKQGESGVSQN--NLGLTLNYRFG

Eca1 DLFN----SGTGYHNPMAVNLGLDYTPVPLVTISAAHKQGESGVSQN--NLGLKLNYRFG

Esp2 DLFR----SGTGYHNPMAVNLGLDYTPVPLLTFSAAHKQGESGVSQN--NLGMKLNYRFG

Esa3 ELFD----SGTGYHNPVAVKVGLSYTPVPLVTVSAHHRQGESGVSQN--DLGLKLNYRFG

Kpn1 DLFH----SGTGYHNPVAVSVGLNYTPVPLVTVTAKHKQGENGVSQN--NVGLKLNYRFG

Pan1 DLFN----SGNRYHNPAALSLGLSYTPVPLVTISASHKTSSAGESQD--QLGLKLNYRFG

Eta2 DLFN----SGNAVADPSAVSLGINYTPVPLFTLAASRKEGEGGESQN--QFTLKMNYRIG

Spr1 DLFG----SGKKQNDPRAMQLGVNYTPVPLVTVKALHKMGEGGVSQD--QVELALNYRLG

Yin1 ALFG----KDNLQKDPSAVTVGVDYTPFPLATLKLIHKRGQQGKNNT--EVGLRVSYQIG

Yin2 ALFG----KDNLQKDPHAVTVGVDYTPFPLATLKVSHKMGKDGKNNT--ELGLQVSYQIG

Yfr4 ALFG----KEHLQKDPRAVTLGLDYTPFPLTTLKVSHKEGQQGQKEA--QVDLQMNYQIG

Ymo2 ALFG----KSHRQKDPRALTLGIDYTPVPLVTLGAKHKYGQQGKKDT--QIDVAFRYQFG

Eco10 ALFG----TDSLEKDPFAVTLGVKYQPVPLIVVGTDFKAGTGDNTDL--SVNATLNYQFG

Eal1 ALFG----TDNLEKDPHAVTLGLNYQPVPLITVGTDFKTGTGDNTDV--TVNATLNYQFG

Sty4 ALFG----TDNLQKDPHAVTLGLEYTPVPLVTVGSDYKAGTGDNNDF--SVKATVNYQIG

Eco16 ALFG----TDNLEKNPQALTVGLMYNPVPLFTVGSDYKSGTGDNSDL--SVNVSLNYQIG

Ahy1 GLLD----ADQLERDPHAITAGLHYNPFPLLKMDVEQVEASGRQHDT--RFTLGLEWKLG

Sen2 DLLG----NGTLSRNPRAAGAALVWNPVPLLEVRAGYRDAGNGGSQA--EGGLRVNYSFG

Efe4 DILG----NDSLTRDPAAFTGAITWKPVPLVEIKAGYKDAGSSGSQT--EAGLNLNYTFG

Bpe1 DYFD----NGRTQAGAKGFKVGVEYRPVSLVSVGLEQTKVIGGGRET--RMQLGLNINLS

Bav2 DYFD----NGRTQDRATGFKYGVQYKPVPLLSLGVEQTRVIGGASQT--SVQLGVALNLS

Bbr1 DYFD----NGRPQRNARGYKYGVEYRPVPLVAVGLEQTKVLGGARQT--TVQLGVNLSLG

Bpa2 DYFD----DGRYRRNPSGFKYGIEYRPVPLIGVGVEQARLQSGERQT--SVQLGVRLNLG

Bav1 DVFD----SGRAQRNASGFRYGVAYQPGALFNIGLNQTRTLDGQKQT--SVQLNVRINLQ

Pma1 QDNS--GLEGAVSWQATPHVGLEAYVSNEISAASTTANTDLPGTDEN--FFGLRMNITGN

Pma3 QDNS--GLEGAVSWQATPHVGLEAWVSNEISAASTTVNTSLPGTDET--FFGLRMNITGN

Pma5 QDNS--GLEGSVRWQATPHLGWEAYVSNEISAASTTVNTSLPGTDEI--FFGLRVNITGN

Pma4 QDNS--GIEGSFNWQATPHVNWEAWVSNEISGAKTVKNSDLPKTDET--FFGLRFRWTAR

Ssp1 SDNS--GIEGSVNWQATPHANLELWVSNEIPAYPTDSNDTIGNQPGP--YIGARVRLTGR

Ssp2 SDNS--GVEGSVNWQATPHTNLSAWISSEIPAYPAQSNAQLSSGDDV--YVGVRFNVQLK

Plu2 NDSN---NPIDDLKGNTFSVSGSVYDGLSVEVGYIDYTSGNADYSKAGGERFLKVSYNFD

Cla1 GIFG-ANSVDDLEKNPLIYEGGISYSPFPALTFTLSHSRSAESSKKN-TSINANINIPLD

Rba1 NWKD----AASYETEHYEAGINAEIAPNLTLRVAAQHKKNSNNTEAV---ASINYSVPLG

13

Mba1 QLFADKPRY----------NHQWISKDAYKLESMEDRRYEKVRRTN--------------

Psp2 NNSN---------------NEIGISDTAYNLTSVSDQKFAKVRRQN--------------

Ybe1 TPWAK----QISP--DAVNARRTLQGSRNDLVERNNQIVLEYKKQD-VINLYV-SNNVSG

Ype5 TPLTH----QLDS--DAVAIKRSLMGSRYNLVDRNNQIVMKYRKQN-RVTLEL-PARVSG

Eco14 EPLAH----QLDS--SRVGAQRSLAANRLELVNRNNDVVLEYRKQT-LITLQL-PPDVYG

Sen3 SSLKS----QLDS--DAVKARRTLLGSRLDLINRNNNIVLEYRKQD-LISLKV-QNKVTG

Ymo1 TPWQK----QIDP--AAVKATRTLAGSRMDLVDRNNNIVLEYRKQQ-VVTLNL-PAKVSG

Yen2 TPWQQ----QIDP--AAVQTTRTLAGRRMDLVDRNNNIVLEYRKQQ-VVTLNL-PEKVSG

Pas1 VPLSQ----QIDP--TVAPVKRTLADNRYHLVERNNNIVLKHRERA-QLSLYL-PTGLSG

Yfr1 VPWVD----QINK--NAVGLMRSLMGSRYDIVDRNYNIVMQYEKQD-LIKLTL-PETLAA

Yps7 ETWQS----HIDP--SAVAASRTLAGSRYDLVERNNHIVLDYQKQN-LVRLSL-PDSLAG

Yfr2 ESWQS----HINP--SAVAATRTLAGSRYDLVERNNNIVLDYQKQE-LIRLSL-PERVEG

Yfr3 EPWQS----QIDP--SAVAASRTLAGSRYDLVERNNNIVLEYQKQD-LIQLVL-PNQMTG

Yps4 QPWND----QIDQ--SAVAANRTLAGSRYDLVERNNNIVLDYKKQE-LIHLVL-PDRISG

Yps2 ESFQS----QLSP--SAVAGTRLLAESRYNLVDRNNNIVLEYQKQQ-VVKLTLSPATISG

Esa2 SSWQS----QISP--EQVATRRSLAGSRYDLVDRNNEIILQYKKK---------------

Plu1 VPLSE----QLSP--ENVSSLRSLAGSRYDLVERNNNIILNYLKKQ-QHFRLL-VPVIEI

Eco1 KPWSQ----QIEP--QYVNELRTLSGSRYDLVQRNNNIILEYKKQD-ILSLNI-PHDING

Pru1 KTWKQ----HLDP--GSVTTFRSLMGNRYDFVSRNNHIVLEYKKND-VIQLNI-ANSITG

Pal3 KSFKQ----HLDP--DAVGEFRSLMGNRYDFVSRNNHILLDYKKND-TIYLNM-NASITG

Eta1 MPWQQ----QINP--QAVATMRSLAGSRYDLVERNNHILLQYRKKE-VIRLHT-VGRVTG

Sgl1 TPWQH----QLDT--SAVGAMRTLAGSRYDLVDRNNNIVLEYRKKE-VIHLYT-ADHLAG

Eco26 TPWES----QINP--VLIPAMRKLAGQRYDLVERNNNIILDYRKKE-IIKIDG-VDVISG

Pmi1 ESLAS----QLNP--DNVKAIRTLAGSRYDFVNRNNDMILEYKKET-LVFLSM-VDSING

Efe5 VPLSK----QLDS--DNVAAMREVQNRRYDFVERNNNIVLEYKKKS-TLRISL-PDAIEG

Eco20 EPLAK----QLDT--DSIRERRVLAGSRYDLVERNNNIVLEYRKSE-VIRIAL-PERIEG

Eco25 SAMQK----QLDP--NEVDARRSLAGSRFDLVDRNNNIVLEYRKKE-LVRLTL-TDPVTG

Eco6 HSLQK----QLDP--AEVAARRSLVGSRYDLVDRNNNIVLEYRKKE-LVRLTL-TDPLKG

Efe2 VSLAR----QLDP--DAVAYRRSLSGSRHDLVERNNNIVLEYRKKE-LVKLQL-NDPVTG

Efe3 VSLAH----QLDP--DAVAYRRSLAGSRHDLVERNNNIVLEYRKKE-LVKLQL-HDPVTG

Pal2 KNLAH----HLDP--TKVAETRRLSGNRYDFVERNNNIILNYQKKT-VLHLSL-PSKIQG

Csu1 ESLSS----QLNP--FSVAKMRFLNENRYNFVSRNHVIVFNYSEYT-YDIFVS-PASISA

Eco3 VPLKK----QLSA--GEVAESQSLRGSRYDNPQRNNLPTLEYRQRK-TLTVFLATPPWDL

Cko1 VPLNK----QLSA--SEVAESQSLRGSRYDNPQRNNLPTMEYRQRK-TLTVFLATPPWDL

Sen1 VPLKK----QLAA--SEVAQSQSLRGSRYDMPQRNALPTMEYRQRK-TLTVFLATPPWDL

Eca1 VPLAK----QLSA--GEVAATRSLRGSRYDPAERNSLPVMEFRQRK-TLSVYLATPPWDL

Esp2 VPLKK----QLSS--EEVATTRSLRGSRYDPVDRTSLPVMEFRQRK-TLSVYLATPPWDL

Esa3 VPLTK----QLSP--DEVAASRSLRGSRYDRVERTNVPVMEFRQRK-TLSVFLATPPWDL

Kpn1 VPLKQ----QLAA--DEVAISNSLRGSRFDSPERDNLPVVEYRQRK-NLTVYLATPPWDL

Pan1 VALSK----QLDA--NNVAEARSLRGSRYDTVTRSQNPVMAYRQRK-TLSVFLATPPWQL

Eta2 VPLRQ----QLSS--DNVAEARSLSGSRYDSVNRDNSPVMAFRQLK-TLSVFLATPPWQL

Spr1 VPLVK----QISP--EYVAQAKSLRGSRYDNIERKNVPVMAFKQRK-TLQVFLATPPWRL

Yin1 TPLDK----QLDP--ANVAAMRSLKGSRYDLVDRNNDIVLEYKEKA-SLALDLAAVPMTL

Yin2 TALEK----QLDP--GNVAAMRSLKGSRYDLVDRNYDIVLEYKEKA-VLSLDLAAVPMTL

Yfr4 TALSK----QLDP--DNVAAMRSLMGSRYDRVDRNYDIVLEYKEKAGLLMLDLAAVPATL

Eco10 VPLKD----QLDP--DKVSAAHSLMGSRHDFVERNNFIVLEYKEKD-PLYVTLWLKADVT

Eal1 TPLKD----QLDP--ENVKIAHSLMGSRHDFVERNNFIVLEYREKD-HLDVTLWLKADAT

Sty4 TPLAA----QLDP--ENVKIQHSLMGSRTDFVDRNNFIILEYREKD-PLDVTLWLKADAT

Eco16 MPLKD----QLDP--DNVKAKHSLMGSRHDFVERNNFIVLEYREQD-PLDVTLWLKADTA

Ahy1 ATLWD----MLNP--SSVD--KSLAGMRHDLIERNNDMVLEYRDKV-LLKASL-NDQYSA

Sen2 TPLHE----QLDY--RNVGAPSNTTN-RRAFVDRNYDIVMAYREQA--SKIRITAMPVSG

Efe4 VPLRA----QLDP--SQVRPASNTTN-RTAFVDRNYNIVMEYREQA--SRIRVYASPVNG

Bpe1 EPLSK----QLRR-DASGTPAFSPDARRHALVERENRIVLNTRRKEIILPLVVSKVSTLQ

Bbr1 EPLSR----QLR---HQSGPAFDLQARMGEFVERENRIVLQTRRKHVVLPLTIARVDTDP

Bpa2 EPLSR----QLRRGAQDTAPPFDRGARLQDFVRRENRIVLDTRRKKIVLALRIAEVRTDP

Bav1 EPPSR----QLRR----ESQPFNLTSRRHQWVERESRIVLNTRRKAITLPLSIAQLRGDP

Pma1 PVKFEK-----------SNYKKNMVTQMTQPVKRKYDVLLERSAG----------GVFQN

Pma3 PIKFEK-----------SNYKKNIITQMTQPVKRVNDVLLERAAINSS-----GAATFTV

Pma5 PVTFGK-----------KDYKKNMITQMTQPVKREYNVLLERYGAN---------STFTN

Pma4 PVKFAK-----------KNLKQNLVTQMTQPVRRRYEVLLERSTGG-----------FQN

Ssp1 PVVFTK-----------SNTKQNLLTQMTQPVRRRYEVLLERVKEPTK-----GENGSIT

Ssp2 PVTYKTG---------SNRIRENLLTQMRQPVQRRNDVLLERWKPKQKKTIGGVNGSFIN

Plu2 IFGTH----------VNKATKPRFSNTPYEFERMDDRRFEKIRFEQKVISAHNNEQPPA-

Cla1 EKAMK---LAFEP--KLAGISNTIEGTRTQFIDRDYSMVLEYRATPNKYHISYCGDLGND

Rba1 GANQP---AKVKQDGDWSTKFEPIREKLYRPVQRENRIMKKAIKLG---------VTVSG

14

Mba1 -----------------------NIVKQIKSLGVKVSGI---------------------

Psp2 -----------------------LIVKQ-KEMDLTVIGF---------------------

Ybe1 RAA------ET-----------KQLVVSV-TSKYGLRNIQFDQGA---------------

Ype5 AAR------QT-----------MPLVANA-TAQQGIDRLEWEASA---------------

Eco14 AEL------TT-----------VTLTPQV-NAKYGLSRIELDDAE---------------

Sen3 TES------ET-----------LPVSVNV-KSKYPLDHISWEDDN---------------

Ymo1 KEK------QV-----------LPINYTF-NARHGLDRIEWDAAD---------------

Yen2 KEK------QV-----------VPINYTF-NARHGLDRIEWDAAD---------------

Pas1 FGG------ER-----------KLINFSF-NGKYRLKHIQWNDGA---------------

Yfr1 YAI------TN-----------LSLTGNI-TAKYGAERMEWSAPA---------------

Yfr5 HAL------EN-----------VTLTGTV-ASKYGTERIEWSAPV---------------

Yps7 DPF------SQ-----------LSVTAQV-TATHGLERIDWQSAE---------------

Yfr2 KAG------DI-----------ATVNAQV-TSKYGLERIDWDSAA---------------

Yfr3 SAF------EI-----------IKVEAQV-TAKYGLKRIDWDTAV---------------

Yps4 SGG------GA-----------ITLTAQV-RAKYGFSRIEWDATP---------------

Yps2 LPG------QV-----------YQVNAQV-QGASAVREIVWSDAE---------------

Esa2 ------------------------------------------------------------

Plu1 SSY------GG-----------EVKPIQI-QSDTPFKNVTWDIPE---------------

Eco1 TER------ST-----------QKIQLIV-KSKYGLDRIVWDDSS---------------

Pru1 YAG------EK-----------IPLSFTV-ASKYGLSHLKWNAET---------------

Pal3 ISG------EK-----------IPLKYTI-NSQYGFNRINWHADS---------------

Eta1 YAG------ER-----------KSLGVSV-NSSYGLERIDWSASS---------------

Sgl1 YAG------EQ-----------KSLNVSI-NTKYGLERIDWSAPE---------------

Eco26 FSG------EK-----------KRLDIRV-NSKYPVDRIDWLANT---------------

Pmi1 YAK------EE-----------RDLQVQV-KTKYPLANIEWSASK---------------

Efe5 ESG------AT-----------IPVTLNVSHASNGIQSVQWSDSA---------------

Eco20 KGG------QT-----------LSLGLVVSKATHGLKNVQWEAPS---------------

Eco25 KSG------EV-----------KSLVSSL-QTKYALKGYNVEATA---------------

Eco15 KSG------EI-----------KPLVSSL-QTKYALKGYNIEAAA---------------

Eco6 KPG------EV-----------KSLVSSL-QTKYALKGYDIEAAS---------------

Efe2 KGG------EQ-----------KPLVASL-QSKYALKTLQTDAAT---------------

Efe3 KGG------EQ-----------KPLVASL-QSKYALKTLRAEAAE---------------

Pal2 ITG------QS-----------VPLVKSF-TSKYPLKHIEWQAPE---------------

Csu1 IEF------AS-----------STVSLKCPNTKT-LAHFEWEAQN---------------

Eco3 KPG------ET-----------VPLKLQI-RSRYGIRQLIWQGDT------------QIL

Cko1 QPG------ET-----------VPLKVQI-RSRHGVRHVTWQGDT------------QAL

Sen1 TPG------ET-----------VTLKLQV-RSVHGIRHLSWQGDT------------QAL

Eca1 KGG------ET-----------VMLKLQI-RSTHGIRQLHWQGDT------------QAL

Esp2 TPG------ET-----------VVLKMQV-RSTHGVRQLHWQGDT------------HAL

Esa3 SAG------ET-----------VALKLQV-RSRHGIRQLSWQGDT------------QAL

Kpn1 QSG------ET-----------VQLKLQI-HSLHGIKALHWQGDT------------QAL

Pan1 NPG------ES-----------VPLKLQL-RASHSIKALSWQGDT------------QAL

Eta2 QPG------ET-----------LRLKLQI-ANKNTIKAVSWQGDT------------QAL

Spr1 QPG------ET-----------LPLVLEI-KTTNKITRVSWQGDT------------QAL

Yin1 LEG------DI-----------YLMQPLV-RSKYKIVGVTWNGDT------------VPL

Yin2 LEG------DV-----------YMMQPLV-RSKYRITSVSWHGDA------------VPL

Yfr4 LEG------DV-----------YLMQPLV-NGKYPIASVSWQGDT------------VPL

Ymo2 MEG------ES-----------YILRPLV-KSKYPIIDLIWLGDL------------LPL

Eco10 NEHPECVIKDTPEEAIGLEKCKWTINALI-NHHYKIVAASWQAKNNAASWQAKNNAARTL

Eal1 NEHPECVIKDTPEEAVGLEKCKWTVNALI-NHHYKIVAASWQAKN---------NAARTL

Sty4 NEHPECVIEDTPEAAVGLEKCKWTVNALI-NHHYKIISASWQAKN---------NAARTL

Eco16 NEHPECIVKDTPELAVGLEKCKWTINALI-NNHYKIVAASWQAKN---------NANRTL

Ahy1 VEG------QA-----------LTLTLNI-QHSRQIASIQWLGDVLG-------------

Sen2 LSG------------------TLVTLMATIDSRYPIEKVEWSGDA---------------

Efe4 QSG------------------DTVTLSATINSRYPVERIEWTGDA---------------

Bpe1 ADG--------------------RVTVIGATQPFATVTVRMPDGM-------------TG

Bav2 VSG--------------------RITLVGQTQAQATVNWTLPDGS-------------SG

Bbr1 ATG--------------------RITVTGVTEPGAQVSLGLPNGE-------------VV

Bpa2 ATG--------------------RITVYGVTEPLADVQLWLPDGT-------------AT

Bav1 TNG--------------------AIEVSGQTEAGARIMLTFPDGS-------------GN

Pma1 R-------------------------------AKGV------------------------

Pma3 RVS-----------------------------AQGT------------------------

Pma5 R-------------------------------AKGS------------------------

Pma4 R-------------------------------ARGI------------------------

Ssp1 S-I-----------------------------AQGI------------------------

Ssp2 Q-------------------------------AAGI------------------------

Plu2 ------------------------------------------------------------

Cla1 KHCILLKN-----------GFDEVVKNTPMRVTPTHSCVVFEKGFD--------------

Rba1 Y-----------------------------------------------------------

15 16

Mba1 ----------------------------

Psp2 ----------------------------

Ybe1 -LAAAGGKIIP----QGPSQFALQLPPQ

Ype5 -LTLAGGKITG----SG-NNWQITLPSY

Eco14 -LRQAGGKIIS----NTGNQITLQLPAW

Sen3 -LVKNGGKISE----NNG-SWSVTLPHY

Ymo1 -VINAGGNISD----QGNLAYHITFPPY

Yen2 -VIKAGGQVIN----QGNLAYYIAMPPY

Pas1 -LRARGGRIIA----LSNNSYVVQFPNY

Yfr1 -LMAAGGSIIP----LTMESASVTLPPY

Yfr5 -LVTTGGALTP----LAMESAVVTLPAY

Yps7 -LMAAGGVLKQ----TSKNGLEITLPEY

Yfr2 -LIAAGGTLSK----GSSNSISITLPPY

Yfr3 -IVAAGGVVTQ----TSSQNISIKLPPY

Yps4 -LENAGGSTSP----LTQSSLSVTLPFY

Yps2 -LIAAGGTLTP----LSTTQFNLVLPPY

Esa2 ----------------------------

Plu1 -LFQKNGGMIN--I-ESTHGYTIQLPEY

Eco1 -LRSQGGQIQHSGS-QSAQDYQAILPAY

Pru1 -LVAAGGHIVQE-----NGKYSLVLPAY

Pal3 -LVAAGGQVIDEK----NGAYSIILPTY

Eta1 -LLAAGGKLVREN----EGSWSVILPEH

Sgl1 -LLAAGGKIVQES----IDNYSIVLPDY

Eco26 -FIANGGKIINEG----LHNYSIILPDY

Pmi1 -LNAQGGQIKHHG----GTHYTVILPQY

Efe5 -FAAAGGKITGS-----GTSWQFTLPAY

Eco20 -LLAEGGKITGQ-----GSQWQVTLPAY

Eco25 -LEAAGGKVVTT-----GKDILVTLPAY

Eco15 -LEAAGGKVSTS-----GKDITVTLPGY

Eco6 -LQSAGGKVAVS-----GKDIQVTIPPY

Efe2 -LTAAGGVISTA-----DNQVTVTLPAY

Efe3 -LQSAGGVVNTE-----ANQVTVTLPEY

Pal2 -FLAVGGSISSD-----DQTATLTLPSY

Csu1 -FIEAGGEITLVSAGKSNAEYTVKLPPH

Eco3 SLTPGAQANSAEG-------WTLIMPDW

Cko1 SLTAGAKADSAEG-------WTIIMPAW

Sen1 SLTAGTDTRNTEG-------WTIIMPAW

Eca1 SLTSPANSTSSDG-------WSIIIPAW

Esp2 SLTSPANANSDEG-------WSVIMPAW

Esa3 SLTPPLDSTSADG-------WTVIMPAW

Kpn1 SLTPPVDASSPDG-------WSIIMPVW

Pan1 SLTSPPNNNAIDG-------WSVIVPPW

Eta2 SLTPPPDNSDPQG-------WSIIIPAW

Spr1 SLTPSQNSNDPHG-------WSLIVPQW

Yin1 SLLATAGATNPQG-------WQITLPAW

Yin2 LLVPTAGANNPQG-------WQITLPAW

Yfr4 ALLATAGVDNHQG-------WQITLPAW

Ymo2 QLLATAGSHNPQG-------WQITLPAW

Eco10 VMPVIKENTLTEGN---NNHWNLVLPAW

Eal1 VMPVIKENTLTEGN---NNRWNIVLPAW

Sty4 VMPVVKADALTEGN---NNSWNLVLPAW

Eco16 VMPVVKANALTEGN---NNRWNLVLPAW

Ahy1 -LSGLSPADTAGQ-----DKRALTLPSL

Sen2 --ELIAGLQLQGS-----LGSGLILPQL

Efe4 --ELIGGLQQQGN-----VNSGLRLPDL

Bpe1 TATADASGRFAYTSAGDQPSGVLALRAR

Bav2 QSRADASGVYRIESRKDQPSGPIRLKAV

Bbr1 VAQADGSGTYRATSARDMVGGPVRARAT

Bpa2 SVRANAAGGFEASSAGDMTSGLIRARAT

Bav1 WVRADASGRYTARSGPDMPSGTVRAQAR

Pma1 ----------------------------

Pma3 ----------------------------

Pma5 ----------------------------

Pma4 ----------------------------

Ssp1 ----------------------------

Ssp2 ----------------------------

Plu2 ----------------------------

Cla1 YITDNSGKVYVQIIRSCTPKTDLNAVAG

Rba1 ----------------------------