CLUSTAL X (1.81) multiple sequence alignment PilY1a_Pae ----------------------------------------MKSVLHQIGKTSLAAALSGA PilY1b_Pae MRGIGTFYYETNSVARNQTNSETVLQTVARPSLYQLIEPRMKSVLHQIGKTSLAAALSGA PilY1a_Xfa -------------VGYFDSNKCYKYHYSNQESDRYFYPVASPRPQANYGCNTAGGVWAGN PilY1b_Xfa -------MSGVKYVGYFDSNKCYKYHYSNQESDRYFYPVASPRPQANYGCNTTGGVWAGN PilC3_Ngo ------------------------------------------------------------ PilC4_Ngo ------------------------------------------------------------ pilC2_Nme ------------------------------------------------------------ pilC1_Nme ------------------------------------------------------------ PilC_Nme ------------------------------------------------------------ Orf_Nme ------------------------------------------------------------ PilC2_Ngo ------------------------------------------------------------ PilC1_Ngo ------------------------------------------------------------ Orf_Xfa --MYFGLLTGSYQKNITGGVLHNNISSFSREINPLTGQFCLNGNCGSGGDVKGIVHTMSS PilY1_Xcac -----------------------------MAVDDSGSMTFQTQFPGADGQGCWSTARESF ComC_Asp ----------------------------------MPVDIMTKDRAGRILAPACKLSTTKA PilY1a_Pae VLLSAQTTHAAALSVSQQP-LMLIQGVAPNMLVTLDDSGSMAFAYAPDSISGYGNYTFFA PilY1b_Pae VLLSAQTTHAAALSVSQQP-LMLIQGVAPNMLVTLDDSGSMAFAYAPDSISGYGNYTFFA PilY1a_Xfa FLNWAATQTIDPFRSALTGGYRVRDTTNETILEKAVMDRDSQENFPRRVVQDRSLLAASV PilY1b_Xfa FLNWAATQTIDPFRSALTGGYRVRDTVNETILEKAVMDRAYPGNFPRRTVQG-GTLAASV PilC3_Ngo ------------------------------------------------------------ PilC4_Ngo ------------------------------------------------------------ pilC2_Nme ------------------------------------------------------------ pilC1_Nme ------------------------------------------------------------ PilC_Nme ------------------------------------------------------------ Orf_Nme ------------------------------------------------------------ PilC2_Ngo ------------------------------------------------------------ PilC1_Ngo ------------------------------------------------------------ Orf_Xfa FRMLDFNYKDYTYGCGWIATRPVKEGECWMWGNPIAEMMYETLRYFGGATAPRPEYDINS PilY1_Xcac FSSAGALNTSGLCGYNHVMVGPRIDTSRLGVPPIDLYGFARSAAYNPTYYNPRVVYDPWI ComC_Asp SDTSNQCFEKGYAGSATTYRGYLVEKVANGIQSKGEFNLQNILLIVPKLVAALVGDVFNK PilY1a_Pae SN-SFNPMYFDPNTQYKLPKKLTLVNGQVQIQDYP-APNFSSAWRNGFTRSGSINLSNSY PilY1b_Pae SN-SFNPMYFDPNTQYKLPKKLTLVNGQVQIQDYP-APNFSSAWRNGFTRRGSINLSNSY PilY1a_Xfa PG-QWDKFRIRIDGLGNRMRFTQATRGLFSFWQEP-LETEGQAYNP-SKHPLNRNDNGVY PilY1b_Xfa PA-EWNSFNIRIDGLGNRMRFTQATRGFFSFWQEP-LETEGQAYNP-SKHPLDRNDNGVY PilC3_Ngo ---------MNKTLKRQVFRHTALYAAILMFSHTG-GGG-AMAQTHKYAIIINNQNQPEV PilC4_Ngo ---------MNKTLKRQVFRHTALYAAILMFSHTG-GGGGEMAKTLKYPIIMNGQNQPKV pilC2_Nme ---------MNKTWKRQVFRHTALYTAILMFSHTG-GGGGAQAQENPYAIVMNSQKLPEV pilC1_Nme ---------MNKTLKRRVFRHTALYAAILMFSHTG-GGG-AMAQTYKYAIIMNEQNQPKV PilC_Nme ---------MNKTLKRQVFRHTALYAAILMFSHTG-GGG-AMAQTHKYAIIMNERNQLEV Orf_Nme ---------MNKTLKRRVFRHTALYAAILMFSHTG-GGGGAMAQTRQYAIIMNERNQPEV PilC2_Ngo ---------MNKTLKRRVFRHTALYAAILMFSHTG-GGGGAQAQTRKYAIIMNERKQPEV PilC1_Ngo ---------MNKTLKPQVFRHTALYAAILMFSHTG-GGGGAMAQTHQYAIVMNERNQPEV Orf_Xfa SSQDIATLQLSHPSWKPPYTSVDQGGSGYSVCAQP-AMTILSDINPSYDDKLPGSHWSNF PilY1_Xcac KEDGKESWGDASTTATRIDPRPTYTATVNLTQNLI-WGDRSDSSRMQNGMVIPVG--TRY ComC_Asp LIGILTNPIRGLTDLLEFENTANVINDLLKTLGLSGNIPTASTYAETGAYLLGTSTKGTG PilY1a_Pae KVTIEYGRGYDKESTIKADAAYYYDFTGSSSCNRTNQACYTRRYVSTEQRQNFANWYSFY PilY1b_Pae KVTIEYGRGYDKESTIKADAAYYYDFTGSSSWNRTNQACYTRRYVSTEQRQNFANWYSFY PilY1a_Xfa EVSVRVKVCDASVGLESNCVAYPSDYYKPEGLIQAYSDRIRYSVFGYKNDHSYLIDGGVL PilY1b_Xfa EVSVRVKVCDATVGLESNCVAYPSGFYKPEGLIQGYSERIRYSVFGYKNMDEYMVDGGVL PilC3_Ngo KQNVQFPP--IKDKNGDRVFNYPTQKNPLGRTNNFISFNNNNTLVSQQSGTAVLGTATYL PilC4_Ngo KQNVPSS---IRDKDRERTYIYNTQSN-YGIS---VSFNNSDELVSQQSGTAVLGTATYL pilC2_Nme KWGNSYKKGELRPEDRDRKFTFTSTYGIKRNTN--ITFNNTDYLVSEKNGTAVFGAATYL pilC1_Nme KQEGSYS--TLREKDRERKFIYNK--GGSGGGS--VSFNNSDELVSQQSGTAVFGTATYL PilC_Nme KGNGQYS--TIKDKDRERKFIYNK--DRGGGGS--VFFDNTDTLVSQQRGTAVFGTATYL Orf_Nme QWNGSYS---IKDKDRKREYTHHN--HQQGGSS--VSFNNSDELVSRQSGTAVFGTATYL PilC2_Ngo KWEGQYSQSTLKDKGRERTFSHTSQRNWNGQQNNFISFNNSDELVSRQSGTAVFGTATYL PilC1_Ngo KWHGQYQKSQLREKDGERKFTYTNQKTGGTNKNNFISFDNTDTLVSRQSGTAVFGTATYL Orf_Xfa SGSADPASMRNLDVSAEADRIWEAEGGGSKLIFIGESNDNSDNAPTPKVVSNLSTVRGLS PilY1_Xcac RVYSGNNLGGTQTANGSEWTAGPADVLISTVRSVFFTPYTSNNDPFPVLPGSVNAYNSVD ComC_Asp ARQIAMRIIPVKILFIPLLGWYDYKCDKYGKDSWQADGVTCKDDAWQYKLGGISTTNLIQ PilY1a_Pae RTRALATQTAANLAFYSLPENARVSWQLLN---DSNCNQMGSGSSSGNCFSNYLRDFTGQ PilY1b_Pae RTRALRTQTAANLAFFRLPENARVSWQLLN---DSNCNQMGSGSRLRQLFQQLSTGLHRS PilY1a_Xfa RARQKFVGPQTHYPEQGKKTNPHAEWDPQTGVLYGNPNPEDAAATTQRVGRTIGNSGVIN PilY1b_Xfa RARQKFVGPQTNYPEQGKKTNLHAEWDPQTGVLYDNPNPEDAAATTQRVGRAISNSGVIN PilC3_Ngo PPYGKVSGFDTDSLK--GRANAVDWIRTTRPGLAGNAYTDVICRSNK-CPQLVYKTQFTF PilC4_Ngo PPYGKVSGFDTDSLK--GRANAVDWIRTTRIALAGYSYAGVICRNITGCPKLVYKTQFTF pilC2_Nme PPYGKVSGFDTDRLT--KRGNAVDWINTTHPGLIGYSYQGITCSSSSSCPEFGYKTQFTF pilC1_Nme PPYGKVSGFDADALK--ERNNALNWINTTRPGLAGYSYANVICRDTRQCPQLFYETKFAF PilC_Nme PPYGKVSGFDADGLQ--KRNNAVDWIHTTQAGLAGYAYTDVICR-SNQCPQLVYETKFSF Orf_Nme PPYGKVSGFDAAALK--ERNNAVDWIHTTHPGLIGYSYDGVVCRSATDCPKLVYKTRFSF PilC2_Ngo PPYGKVSGFDADGLK--KRGNAVNWIRTTRPGLAGYIYTGVICRDTGQCPELVYETKFSF PilC1_Ngo PPYGKVSGFDIAELN--KRGNAVNWIHTTRAALSGYSYTGVTCRSGKCRPKLVYKTRFSF Orf_Xfa PEEPSKGGTYYSAAVARYGANHKMGGTKFVRTYAVALASPLPKFEFPVGNARISLVPFAK PilY1_Xcac TPRIRVANACGQGCDLWKYTINASSTDALRNFANWFSFYGNRNKAIVAGLTRSLVNVRQD ComC_Asp VNDDLVSSLISDGVVGGLLNGVVGNILGIKVSEKHLYYGYDSQADIEYSGFNYRDNSVPT PilY1a_Pae HRVNFFNWLEKLSVNGGTPLRQAMTRAGEFLKKTGVNGPYAYRPG-----TQTAPEYSCR PilY1b_Pae TAGELLQLAGKTFGQWWYALRQAMTREASFSRRPASNGPYAYRPG-----TQTAPEYSCR PilY1a_Xfa YLNKFGQMETGRALKIYDPVSELYYAAFRYFKGLGNVPEYSALSGSANEKYQQADAFPVI PilY1b_Xfa YLNKFSQMETGKNTKGFDAVSELYYTAIRYFKRLGNVPEYSVLTGSVTQRYQQADAFPVI PilC3_Ngo GQQGLQR-KD-SKLDIYEDKSRENSPIYKLKDYPWLGVSFNLGSEGTAKDGRVTNKLVYS PilC4_Ngo DQQGLKK-KAGSKLDIYEDKSRENSPIYKLSDYPWLGVSFNLGSENTVKDGKSFNKLISS pilC2_Nme GNSGLAKRQTSSKLDIYEDKSRDNSPIYKLQDYPWLGVSFNLSSDTYVKQ-KRHNSLVSS pilC1_Nme SNKDLVN--NAGRLDRHPDPSRENSPIYKLKDHPWLGVSFNLGSEGTAKDGKVSNKLISS PilC_Nme DGIGLAK--NAGSLDRHPDPSRENSPIYKLKDHPWLGVSFNLGSENTVKDGKSFNKLISS Orf_Nme DNPDLAK--TGGGLDKHTEPSRDNSPIYKLKDHPWLGVSFNLGAEGIAKNGKTINKLVSS PilC2_Ngo DGIDLAK-GGNRKLDRHPDPSRENLPIYKLKDHPWLGVSFNLGGEGTAKDGRSSSRWISS PilC1_Ngo DNTGLAK-NAGR-LNRHPDPSRENSPIYKLEDHPWLGVSFNLGAEGTDKDGRSSNKLVSS Orf_Xfa SVHGYGISAAGNFQPTNQIIDFYVQRVANMAGSSGADYDATINGGRPYAEFRINYEDVEQ PilY1_Xcac LRVGYFRINQHASFDSVSDALPMRTMGNDTQRKALYAEMIALTASGGTPNREAVNAAGIQ ComC_Asp RNNNQFYQAPANQPQCNASGIMVITGGVPNITPTTTDVLLQPSGKEGLGTKNAIERLMGR . PilY1a_Pae GSYHILMTDGLWNNDSANVGNADSTARNLPDGKSYSSQTPYRDGTFDTLADQAFHYWATD PilY1b_Pae GSYHILMTDGLWNNDSANVGNADSTARNLPDGKSYSSQTPYRDGTFDTLADQAFHYWATD PilY1a_Xfa TDWGDPIRYACQSNVVLGIGDTHTNQDKNLPGNTNSMEEPAKPQAVKNDRSVDVVKRMTQ PilY1b_Xfa TEWDDPIRYACQSNVVLGIGDTNTNRDKNLPGSTSNMEEPAKPQAVKNDRSVDVVTRMTQ PilC3_Ngo FDEKNS----------NNNLVYTTEG-RNISMGN-QRESTAMAYYLNAKLHLLDKKKIEN PilC4_Ngo FGEDNN----------NQTIVSTTED-YPISLGDWQREHHAMAFYLNAKLHLLDKKGIQN pilC2_Nme FSEDVTQSNGTQSPYKDRNLVYRTDDYHNKGNYSHQDAHHAIAFYLNAKLHLLDKKQIKN pilC1_Nme FSEDNN----------NQTIVSTTRD-HPISLGDQQREHTAMAYYLNAKLHLLDKKGIKD PilC_Nme FSEGNN----------NQTIVSTTRG-HSISLSDWKREHTAMAYYLNAKLHLLDKKGIED Orf_Nme FNEKNS----------NNNLVYTTEG-RDISLGNWQRETTAMAYYLNAKLHLLDKKQIQN PilC2_Ngo FSEDNN----------NQTIVFTTRG-HPISLGDWQRESTAMAYYLDAKLHLLDKTQIEN PilC1_Ngo FSDNNS----------NQIIVCTTEG-HRISLGDWQREHTAMAYYLNAKLHLLDKKQIEN Orf_Xfa GADHDMDAIVLYTLSVNAKNQLVVTLKSEYSAGSIDQHMGYVISGTTRDGVYLEICDLAD PilY1_Xcac FQRTDTGSPIQFACQKNAVMLFTDGYNNNPGGSAGATNAQGDGGMGPPFSDTYDNTMGDI ComC_Asp SLDTQATDLSRLDLQSVFQCDESAGLKSTTLSSRDYATWSCIGNYSKKLLDKNVSTGVIG PilY1a_Pae ARPDIDDNIKPYIPYPDQANPSAEYWNPRNDPATWQHMVTYTLGLGLT-TSLTSP--RWE PilY1b_Pae ARPDIDDNIKPYIPYPDQDNPSGEYWNPRNDPAIWQHMVTYTLGLGLN-TSLTSP--RWE PilY1a_Xfa IFQMEGMSQRDAMAAAVAPKFNFHRYNSAYIAALAYDAHTKDMRPDLEGDQLLTT--HWV PilY1b_Xfa IFYMEGMSARNAVNAARASNFNAN-SNSAYIAALAYDAHTKDMRPDLEGDQLLTT--HWV PilC3_Ngo IAPGKTVDLGTLRP----RVET-TGRSWLDFWATWKIEDKGNITVRLGLPEVKAG--RCI PilC4_Ngo IAPGKTVRLGVLKPSIDVRTRN-TGFGFLNFWAKWDIKDNGQIPVKLGLPEVKAG--RCI pilC2_Nme IAQGSELNLGMLKTHIELPKNF-NQRSLVGSVVSWTVKDEGKVSVKLKLPEVKAG--RCI pilC1_Nme IAQGKTVDLGTLRPRVETKTN--NWGNLLNFWVTWKIEDNGQIPVKLGLPEVKAG--RCT PilC_Nme IAQGKTVDLGTLRPRVEATVR---RGELLNFWATWKIEDKGNITVRLGLPEVKAG--RCV Orf_Nme ITD-KTVQLGVLKPSIDVRTRNTGTAGILSYWAKWDIKDTGQIPVKLSLTQVKAG--RCV PilC2_Ngo IAPGKTVNLGILRPRVEAKVRR--KWDLLNFWAKWDIKDTGQIPVKLGLPEVKAG--RCI PilC1_Ngo IAPGKTVQLGTLRPRVETTGR-----SWLDFWATWKIEDKGNITVRLDLPEVKAG--RCI Orf_Xfa GHSNNGTRSSCAGQQPYKLNTPPNRLPGYCNATPMPGDCNGLPPVATRIFSVASQGANAV PilY1_Xcac AAKYYNDVAGRSPLRPDLPAGLVPVPSACAAAPSVRLDCQKNLHINFYGVTLGARG-NLF ComC_Asp VGREFITIPSTKDSAQLEASMSNLNNSLLGQTVPNLVKDTLNVLLGGPLVNGLTNLLGNL : PilY1a_Pae GSTFSGGYNDIVAGNLSWPRASNN--DSNNVYDLWHAAVNSRGEFFSADSPDQLVAAFQD PilY1b_Pae GSTFSGGYNDIVAGNLSWPRASNN--DSNNVYDLWHAAVNSRGEFFSADSPDQLVAAFQD PilY1a_Xfa DVVEAGDYKSPISTNQYWLAAKYGGFQVPAGYDPDKTVKPLSEETWWTNG-EYVNNDLKA PilY1b_Xfa DVIEGGSFKTPIT-NQYWLATKYGGFQVPAGYDPDKTANALPEATWWTNG-EYVNND--S PilC3_Ngo NKPNPNPKS-ALSPALTAPALWFG------------PVQNGKVQMYSASVSTYPDSSSSR PilC4_Ngo NKPNPNKNTKAPSPALTAPALWFG------------PVQNGKVQMYSASVSTYPDSSSSK pilC2_Nme NKDNPNPKSKAPSPALTAPALWFG------------PVQNGKAQMYSASVSTYPNSCSSR pilC1_Nme NAAHPNSKAKPPSPALTAPALWFG------------PVQNGKVQMYSASVSTYPGSSSSR PilC_Nme NKANPNPNAKAPSPALTAPALWFG------------PVKDGKAEMYSASVSTYPDSSSSR Orf_Nme NKDNPNKNTKTSSPALTAPALWFG------------AGQDGKAEMYSASVSTYPDSSSSR PilC2_Ngo NKPNPNPKS-ALSPALTAPALWFG------------PVQNGKVQMYSASVSTYPGSSSSR PilC1_Ngo NKPNPNKNTKAPSPALTAPALWFG------------PVQNGKVQMYSASVSTYPGSSSSR Orf_Xfa LLKDPLWYAAKYGHDQGVILNSSGGLANYFPVNNALYLRQQVAKAFSAIQSQAGSSGSIA PilY1_Xcac NPDIVQDAYTDAAIYRNWPARSDN--DPATVDDIWHAAINTRGDYINARTPADVTAAIDR ComC_Asp FPTDAEDVKNLARWGVLGKGGWYNSASSENIANSVYSFYSDLGVTKRETFLGAPVIPTDP . PilY1a_Pae ILNRISGKDLPASRPAISSSLQEDDTGDKLTRFAYQTS-FASDKNWAGDLTRYSLTTQDK PilY1b_Pae ILNRISGKDLPASRPAISSSLQEDDTGDKLTRFAYQTS-FASDKNWAGDLTRYSLTTQDK PilY1a_Xfa NAKRADNFYVAADAEKMVASLK-----QAFSRIVAETK-GAGTGLSSNSARLETGAVTYQ PilY1b_Xfa AFKRADNFYIAADAEKMVASLK-----HAFSRIVADTK-GAGTGLSSNSARLETGAVTYQ PilC3_Ngo IFLQELETQTEPGKPGRYSLKP-------LSENEIKVK-EPRFNRRQTVIRLDSGVQLIN PilC4_Ngo IFLQNLSRNDDKNKPGRYSLQP-------LNENEIKSQ-EPSFTIRQTIIRLDDGVHLIR pilC2_Nme IFLQNSERKDDPNKPGRHSLAT-------LTENDIKNR-EPTFNQRQTIIRLDGGVQQIK pilC1_Nme IFLQELKTQTDPGRPGRYSLAA-------LDAQNIKSH-EPRFNGRQTVIRLPGGVYKIS PilC_Nme IYLQNLKRKTDPGKPGRHSLET-------LTENDIKSR-EPNFTGRQTIIRLNGGVREIK Orf_Nme IFLQNLKRKTDTSRPGRYSLAT-------LNKSDIESR-EPSFTSRQTVIRLDGGVQQIK PilC2_Ngo IFLQELKTKTDPARPGRHSLAA-------LNAQDIKSR-EPNFNSRQTVIRLPGGVYQIA PilC1_Ngo IFLQELKTQTDPARPGRHSLAA-------LNAQDIKSR-EPNFNSRQTVIRLPGGVYKIS Orf_Xfa VVGASVSSTSFAVIPSYSSTHDGKDWTGEMTAYRIDANGMLGDVLWSASAGVPSGTSAIA PilY1_Xcac VITSVTAGESPSGTAAQTGARIG----NGSFAVAPAYQIGTDGTDWYSRLIGSRVSLDPQ ComC_Asp LTPYNLNNYVFQNMFVPDDKQT------WFGNVKKYMTDTGETIQTKTFQDVWSNQNIDQ PilY1a_Pae ATVQTKLWSAQSILDAMPNGGAGRKIMMAGSGTSGLKEFTWGSLSADQQRQLNRDPDRND PilY1b_Pae ATVQTNLWSAQSILDAMPNGGAGRKIMMAGSGTSGLKEFTWGSLSADQQRKLNRDPDRND PilY1a_Xfa AQFFSGTWRGDLIAYHVDKVTGALTPFWN-ANFPAWEQRVITFANGTTLQDFTKKNLGET PilY1b_Xfa AQFFSGTWRGDLIAYHVDKVTGALTPFWN-ANFPAWEQRVITFANGTTLQDFTKKNLGQT PilC3_Ngo LNWNNDEVVTFG----GNAGNNGTFGIVKEADVNLEADEWKKVLLPWTVRAPGNDNKFKS PilC4_Ngo LSRARDEVIDFVNLNGNNTGKNDTFGIVKEANVNLNADEWKKVLLPWTVRGPDNDNKFKS pilC2_Nme LDKSN-EATGLN----GNTN-NNTFGIVKDLGVDPDANEWKKVLLPWTVRAFNDDGRFNT pilC1_Nme LGRNGGRVAGIN----GNDGKNDTFGIFKDRYVTPEADEWSEMLLPWTVRAFNDDGRFNT PilC_Nme LDRNNTEVVNFN----GNDGNNDTFGIVKDLGVEPDTSEWKKVLLPWTVRGFADDNKFKA Orf_Nme LDRNNTEVTGFN----GNDGKNDTFGIVSEGSFMPDASEWKKVLLPWTVRAFNYDGRFNT PilC2_Ngo PG-NSGRVAGFN----GNDGKNDTFGIYKDRLVTPEVGEWSEVLLPWTARYYGNDDIFKT PilC1_Ngo SGKNGGRVAGIN----GNDGKNDTFGIYKDKLVTPEADEWSNILLPWTARYYGNDDIFKT Orf_Xfa KRVIYTALSHVDDTNRASVVRRFVAEKLVESSSGDVATDAAQVFGRFGYTPRGVIDDFGS PilY1_Xcac SRLPITTPVWEASARLAAMTPASRNIIVNEAGVAAAFNDTSISLADLCSKPAGLYPSMSK ComC_Asp TNILSGGVIDKLPIASGNNASTRQLYINRTCDTKAKKYDVSNAISAVGNNYYTQLCAGQT PilY1a_Pae VADT-------KGQDRVAFLRGDRRKEN--SDNFRTRNSILGDIINSSPATVGKAQYLTY PilY1b_Pae VADT-------KGQDRVAFLRGDRRKEN--SDNFRTRNSILGDIINSSPATVGKAQYLTY PilY1a_Xfa ALAS-------ASAQQINYLRGDRSQEGNVPGKLRIRSGIMGDIVNSQPLYVGAPNGRLY PilY1b_Xfa ALAS-------ASAQQINYLRGDRSQEGNVPGKLRIRSGIMGDIVNSQPLYVGAPNGRLY PilC3_Ngo INRE-------PGK-------YSQRYRI--RDNNGN--RDLGDIVNSPIVAVGG----YL PilC4_Ngo INRE-------SDK-------YSQRYRI--REN-GN--RDLGDIVNSPIVAVGG----YL pilC2_Nme VNKE-------ENN---GKPKYSQKYRS--RDNGKH-ERNLGDIVNSPIVAVGE----YL pilC1_Nme VNKE-------ENN---GKPKYSQRYRI--RENGNNGKRDLGDIVNSPIVAVGG----YL PilC_Nme FNKE-------ENND--NKPKYSQKYRS--RDNNKG-ERNLGDIVNSPIVAVGE----YL Orf_Nme VNKE-------ENN---GKPKYSQKYRS--RNNGKH-ERNLGDIVNSPIVAVGE----YL PilC2_Ngo FNQP-------NSKTQNGKKQYSQKYRIRTKENDNDKPRDLGDIVNSPIVAVGG----YL PilC1_Ngo FNQP-------NNKKQSDKKQYSQKYRIRTKEDDNDN-RDLGDIVNSPITAVGG----YL Orf_Xfa SVTP-------NQLVNYLRGDKSMEGTTLNTAPFRRRFGPLGDMINSIPVVATRRANYGW PilY1_Xcac CSEAGLTSIGATARTAIAYLRGDPANEVRNSGGFRDRTSRLGDIINSAPVISAPMDDYGY ComC_Asp TDPR-------RNDLMNLLGYQIQSVNNQETLVAKPEYRKIGMVLHSTPIKITQSATVKD :* :::* PilY1a_Pae LAQPIEPSGNYSTF-----AEAQKTRAPRVYVGANDGMLHGFDTD-GNE----------T PilY1b_Pae LAQPIEPSGNYSTF-----AEAQKTRAPRVYVGANDGMLHGFDTD-GNE----------T PilY1a_Xfa TTASFTGASAYAAF-----VAQQANRAPVVYVGANDGMLHAFDANTGKE----------I PilY1b_Xfa TTASFTGASSYAAF-----VAQQANRAPVVYVGANDGMLHAFDANTGKE----------I PilC3_Ngo ATAANDGMVHIFKK-----NGGSDERSYNLKLSYIPGTMP--RQYFDND----------T PilC4_Ngo ATAANDGMVHIFKK-----NGGSDERSYNLKLSYIPGTMG--RKDIEN------------ pilC2_Nme ATSANDGMVHIFKQ-----SGG-DKRSYNLKLSYIPGTMP--RKDIQN------------ pilC1_Nme ATSANDGMVHIFKQ-----SGG-DKRGYNLKLSYIPGTMP--RKNIEN------------ PilC_Nme ATSANDGMVHIFKQ-----SGG-DKRSYNLKLSYIPGTMP--RKDIQN------------ Orf_Nme ATSANDGMVHIFKQ-----SGG-DKRSYNLKLSYIPGTMP--RKDIES------------ PilC2_Ngo ATSANDGMVHIFKK-----TGT-DERSYNLKLSYIPGTME--RKDIEG------------ PilC1_Ngo ATSANDGMVHIFKK-----NGT-DQRGYELKLSYIPGTMP--RKDIES------------ Orf_Xfa ATASGLPQVQRDSYSGFINARQNSTAAEHIFVGANDGMLHAFDDK-GTE----------R PilY1_Xcac RALPDALGTTYGSY----LSAKSASGRYTVYVGANDGMLHAFDAGMTADGSMDASGGLER ComC_Asp DGSLTRDDYLVFGSAEGLLHVVNADTGVEKLAFLPNEMLENSKQRKAFTG--NGLEGHDS : PilY1a_Pae FAFIPSAVFEKLHKLTARGYQGG-----AHQFYVDGSPVVADAFFGG-AWHTVLIG-SLR PilY1b_Pae FAFIPSAVFEKLHKLTARGYQGG-----AHQFYVDGSPVVADAFFGG-AWHTVLIG-SLR PilY1a_Xfa FAFVPRAAMPKLLEYTDQNYV--------HQYYVDGELTAADIYDTKSGWRSVLVG-TLG PilY1b_Xfa FAFVPRAAMPKLLEYTDQNYV--------HQYYVDGELTAADIYDTKSGWRSVLVG-TLG PilC3_Ngo SALKDSTLAQELRTFAEKGYVG-------DRYGVDGGFVLRQVN--LNGQDRVFMFGAMG PilC4_Ngo ---QESTLAKELRAFAEKCYVG-------DRYGVDGGFVLRRITDDQDRQKHFFMFGAMG pilC2_Nme ---TESTLAKELRAFAEKSYVG-------DRYGVDGGFVLRKVE--RNGKDHVFMFGAMG pilC1_Nme ---NDSTLAKELRAFAEKGYVG-------DRYGVDGGFVLRRITDDQDKQKHFFMFGAMG PilC_Nme ---TESTLAKDVRTFAEKGYVG-------DRYGVDGGFVLRKVDN-LNGQNRVFMFGAMG Orf_Nme ---KDSTLAKELRAFAEKGYVG-------DRYGVDGGFVLRRITDDQDKQKHFFMFGAMG PilC2_Ngo ---NDSDLAKELRTFAEKGYVG-------DRYGVDGGFVLRRITDDQDKQKHFFMFGAMG PilC1_Ngo ---NDSTLAKELRAFAEKGYVG-------DRYGVDGGFVLCPLQMTS-QAEHFFMCGAMA Orf_Xfa FAYVPNGVMHHLGFLANPEYQH--------HYYVDGKLTLSDAYLNG-SWKNVLVG-GTG PilY1_Xcac FAYIPATALGHMGNLLLPSDPTNRTAEYEHRYYVDGPVVVGDANYAG-SWKTVVVG-AAG ComC_Asp FSNMQYGVDGPWTAYTEYVWNSKDNKLTVGKSTTDAVCIKDGVFTGACGKQYLYGG--LR : .*. . PilY1a_Pae AGGKGLFALDVTDPA------NIKLLWEIGVDQ----------EPDLGYSFPKPTVARLH PilY1b_Pae AGGKGLFALDVTDPA------NIKLLWEIGVDQ----------EPDLGYSFPKPTVARLH PilY1a_Xfa RGGKGLFALDVTDPS------NIRLLWDKTSAE----------IGGLGNTLSKPMIVQTS PilY1b_Xfa RGGKGLFALDVTDPS------NIRLLWDKTSTE----------IGGLGNTLSKPMIVQTS PilC3_Ngo LGGRGAYALDLSKIN--GNYPAAAPLFDVKDGD---NNGKNRVEVKLGYTVGTPQIGKTQ PilC4_Ngo LGGRGAYALDLSKID--GNYPAAAPLFDVKDGD---KKGKNRVEVKLGYTVGTPQIGKTQ pilC2_Nme FGGRGAYALDLSKIDSGNGNLADVSLFDVKHD----KNGNN--GVKLGYTVGTPQIGKTH pilC1_Nme FGGRGAYALDLTKAD--DNDPTKASLFDVKDNGNNGNNGNN--RVELGYTVGTPQIGKTH PilC_Nme FGGRGAYALDLTKAD--GSDPTAVSLFDVKDNG---NNGNN--RVELGYTVGTPQIGKTH Orf_Nme LGGRGAYALDLTKAD--DNDPTKASLFDVKDNGNNGNNGNN--RVELGYTVGTPQIGKTH PilC2_Ngo LGGRGAYALDLSKIDS-SNL-TGVSMFDVQNDKNNNNNKNDNNRVKLGYTVGTPQIGKTQ PilC1_Ngo LGGRGVYALDSRKIND-FFFPVGRPLFGVKDGDNNGKNR---VKVDLGYTRRTRNFGTPP Orf_Xfa AGGRSMFALDVNSPS---TFNESNVLWEMNSQN----------DDDMGYTMGKPYIVPLQ PilY1_Xcac AGGRSIFALDVTNPS---NFSTSSKLWEISDLDS---SLSTAIRNNIGHVLGKPVIVPVK ComC_Asp MGGRSYYALDLNDLAAPKLKFYIDPASGRVYSDAYPGGKSFDAIKNMGQSWSKPTIAWIN **:. :*** . :* . . PilY1a_Pae N----GKWAVVTGNGYSS--------------------LNDKAALLIIDLETGAITRKLE PilY1b_Pae N----GKWAVVTGNGYSS--------------------LNDKAALLIIDLETGAITRKLE PilY1a_Xfa D----GTWSVLLGNGPNS--------------------TADNAQLIVMNLLTGHATQVVV PilY1b_Xfa D----AVWSVLLGNGPNS--------------------TADNAQLIVMNLLTGHATQVVV PilC3_Ngo N----GKYSAFLASGYAAKDIDD---------------PTNKTALYVYDLKDTL-GTPIA PilC4_Ngo N----GKYSAFLASGYAAKHIAS---------------PTNKTALYVYDLGNTL-GTPIA pilC2_Nme D----GKYAAFLASGYATKDITS---------------GDNKTALYVYDLESS--GTLIK pilC1_Nme N----GKYAAFLASGYATKKIDD---------------PTNKTALYVYDLKNNN-GTPIA PilC_Nme D----GKYAAFLASGYATKEIITS--------------GDNKTALYVYDLEGNGTNNLIK Orf_Nme N----GKYAAFLASGYATKQIDS---------------GENKTALYVYDLESNN-GTLIR PilC2_Ngo N----GKYAAFLASGYAAKNIGS---------------GDNTTALYVYDLENTS-GSLIK PilC1_Ngo KR---GKYSVSLGSGYWGKNIGS---------------GDNKTALYVYDLGNTL-GTPIA Orf_Xfa D----GSWAAIFGNGYNS--------------------SNGRAVLFIVNLATGQLIRKIE PilY1_Xcac NAAGAVTWKAIFGNGYNS--------------------RNGKAVLFVVDIKTGAPAVTMI ComC_Asp WQ-GKRKLVMFVGGGYDAGGTDGNSNNGGYELANYNQTNKRGAGVYMFDAENGDLLWWAN ..* : : : : PilY1a_Pae VTGRTG---------VPNGLSSPRLADNNS---------DGVADYAYAGDLQGNLWRFDL PilY1b_Pae VTGRTG---------VPNGLSSLRLADNNS---------DGVADYAYAGDLQGNLWRFDL PilY1a_Xfa SKASS------------NGLSGVLPWSSQS---------NGITDRVYAGDLLGTLWRFTF PilY1b_Xfa SKASS------------NGLSGVLPWSSQS---------NGITDRVYAGDLLGTLWRFTF PilC3_Ngo KIEAPG---------GKGGLSSPTLVDKDL---------DGTVDIAYAGDRGGNMYRFDL PilC4_Ngo KIDVPG---------GKGGLSSPTLVDKNL---------DGTVDIAYAGDRGGSMYRFDL pilC2_Nme KIEVPN---------GKGGLSSPTLVDKDL---------DGTVDIAYAGDRGGNMYRFDL pilC1_Nme KIEVPN---------GKGGLSSPTLVDKDL---------DGTVDIAYAGDRGGNMYRFDL PilC_Nme KIEVPG---------GKGGLSSPTLVDKDL---------DGTVDIAYAGDRGGNMYRFDL Orf_Nme KIEVTD---------GKGGLSSPTLVDKDL---------DGTVDIAYAGDRGGKMYRFDL PilC2_Ngo KIEAPG---------GKGGLSSPTLVDKDL---------DGTVDIAYAGDRGGNMYRFDL PilC1_Ngo KIDVKD---------GKGGLSSPTLVDKDL---------DGTADIAYAGDRGGYMYRLDL Orf_Xfa VRDGIDPDGSDPANMGYNGLGNLAVLDTDG---------DGLVDMVYGADLHGNLWKFNL PilY1_Xcac EATETG-----STIAGSNGLGNIVVVDRWGGASQTDGVRDGYADTVYGADQKGAIWKFDL ComC_Asp NLATTSN------DVSNSALNTNMQFSVVGRINTVDRDGDGLIDHLYFGDLGGQLWRVDI ..*. . :* * * .* * :::. : PilY1a_Pae IAGKVNQDDPFSRANDGPAVASSFRVSFGGQPLYSAVDSAGAAQAITAAPSLVRHPTRKG PilY1b_Pae IAGKVNQDDPFSRANDGPTVASSFRVSFGGQPLYSAVDSAGAAQAITAAPSLVRHPTRKG PilY1a_Xfa SDNAWKVAPLFTATYQG------------------------KAQPISATPLGAIERSTGR PilY1b_Xfa SDNAWKVAPLFTATYQG------------------------KAQPISATPLGAIERSTGR PilC3_Ngo SNSDPNKWSAKAIFEGT--------------------------KPITSAPAVSRLKDKR- PilC4_Ngo SGDNPSKWTVRTIFQGT--------------------------KPITSAPAVSRLADKR- pilC2_Nme SNNDPTKWSVRTIFKGTP------------------------DKPITSAPAVSKLKDKR- pilC1_Nme SNNDPTKWSVRTIFKGTR------------------------DKPITSAPAVSKLKDKR- PilC_Nme SSQDPQQWSVRTIFEGT--------------------------KPITSAPAISQLKDKR- Orf_Nme SGNNPNSWTVRTIFQGT--------------------------KPITSAPAISQLKDKR- PilC2_Ngo SNSDSSKWSAKVIFEGD--------------------------KPITSAPAVSRLADKR- PilC1_Ngo SNSDPSKCSVSTIFEAA--------------------------KPITSVAAVSRLADKR- Orf_Xfa SGKDPQRWGIAYKDGLGN-----------PIPLFVARNPQGYRQPITGGLEVAVGPSAG- PilY1_Xcac RSSAASVTVPFFTTRTSVE------------------SGQTYRQPITGGLVATAGDAGG- ComC_Asp NNRVDAKQFASAALLLDLQ-------------SNRPTDLKDTNVRFYEAPVFSIYGYGSE : PilY1a_Pae YIVIFGTGKYFENADARADTSRAQTLYGIWDQQTKGEAAGST-PRLTRGNLQQQTLDLQA PilY1b_Pae YIVIFGTGKYFENADARADTSRAQTLYGIWDQQTKGEAAGST-PRLTRGNLQQQTLDLQA PilY1a_Xfa MWIFFGTGRVLS--SHDMDNKEVQSWYGLIDQGTTIPGR--------TGLSQVQIVDEGV PilY1b_Xfa MWIFFGTGRVLS--SHDMDNKEVQSWYGLIDQGTTIPGR--------TGLSQVQIVDEGV PilC3_Ngo -VVIFGTGSDLT--EDDVLDTKEQYIYGIFDDDKGTGTVKVTVQNGTAGGLLEQNLMQEN PilC4_Ngo -VVIFGTGSDLS--EEDVVGTDQQYIYGIFDNNK--GTVKVTVQNGTGGGLLEQTLTKES pilC2_Nme -VVIFGTGSDLS--EDDVDKKDIQSIYGIFDNDTGTDVAE----EGQGKGLLEQHLTQED pilC1_Nme -VVIFGTGSDLS--EDDVDKKDIQFIYGIFDNDTGTGSAE----GGQGKGLLEQHLTQED PilC_Nme -VVIFGTGSDLS--EEDVDNMEEQYIYGIFDDDTATTGTVN--FSDSGGGLLEQVLRRDN Orf_Nme -VVIFGTGSDLS--EDDVLSTDEQHIYGIFDNDTNTGTAQ----EGLGKGLLEQKLSEEN PilC2_Ngo -VVIFGTGSDLS--EQDVLDTDKQYIYGIFDDDKSTVNVKVT--NGTGGGLLEQVLKEES PilC1_Ngo -VAIFGTARYLT--EEDVLDRKEQYIYGIFDDDKGTVKVTVQ--NGTAGGLLEQVLKEEN Orf_Xfa YIIYFGSGRYFA--ANDNNSKDINTLYGIWDSGSAVIAG--------RAALSAQIIQVSD PilY1_Xcac VMLFFGTGSFSF--ESDPKDEAIQSLYGVNDTVRGAPLTTAT-----RANLRGSTVVQDG ComC_Asp SLAVLSIASSNR--SLPISDKSAGAIFNIFDKDVTQVSFTTRDTYTDNKNLVAYSQLPKW :. . :.: * PilY1a_Pae DSTFASTARTIRIASQNPVNWLNNDGSTKQSGWYLDFMVN--GTLKGEMLIEDMIAIGQV PilY1b_Pae DSTFASTARTIRIGSQNPVNWLNNDGSTKQSGWYLDFMVN--GTLKGEMLIEDMIAIGQV PilY1a_Xfa VNGYAVRT-------------VSDPKNIGTDGWYMDLISPKSGKQGERMIVSNMFRGAAL PilY1b_Xfa VNGYAVRT-------------VSDPKNVGADGWYMDLISPKSGKQGERMIVSNMFRGAAL PilC3_Ngo --KTLFLN--KRSDGSGSKGWAVKLTGGQRVTVKPTVVLRTAFVTIRKYN-DGGCGAETA PilC4_Ngo --NTLFLSNNKASGGSNGEGWVVKLGEGERVTVKPTVVLRTAFVTIRKYTGNDKCDAQTA pilC2_Nme --KTLFLTDYKRSDGSGDKGWVVKLEAGQRVTVKPTVVLRTAFVTIRKYN-DGGCGAETA pilC1_Nme --KTLFLTDYKRSDGSGDKGWVVKLEAGQRVTVKPTVVLRTAFVTIRKYN-DGGCGAETA PilC_Nme DNKTLFLTDYKRSDGSGNKGWVVKLKDGQRVTVKPTVVLRTAFVTIHKYTGTDKCGAETA Orf_Nme --KTLFLTDYKRSDGSGDKGWVVKLKDGQRVTVKPTVVLRTAFVTIHKYTGNDKCGAETA PilC2_Ngo --KTLFLSNNKASGGSADKGWVVKLREGERVTVKPTVVLRTAFVTIRKYTDTDKCGAQTA PilC1_Ngo --KTLFLN--KGSVGSQSKGWIVKLKEGQRLTDKPTVVLRTALVTIRSYTGADNCGAQPA Orf_Xfa HPTSPDTR----------IVTRRPLSYLTKHGWYVDLVVQGQDPQGERSIATPLLQGGRV PilY1_Xcac -----------------DRTLSRAAAPANSQGWYVDLPAK------ERMVGSPSIASGIV ComC_Asp G--------------------ALTNENKPQYGWYVKLIDQQKVMDETAVINKSLYVS--- . PilY1a_Pae VLLQTITPNDDP-CADGASNWTYGLDPYTGGRTSFTVFDLARQGVVDSK--------SDY PilY1b_Pae VLLQTITPNDDP-CADGASNWTYGLDPYTGGRTRFTVFDLGRQGVVG------------- PilY1a_Xfa IGTTRIPDNSDI-CKLSGSGFVMAINPFTGGRLGQWFFDLNTGGGSGGSGGSGGSGGSGG PilY1b_Xfa IGTTRIPDNSDI-CKLGGSGFVMAINPFTGGRLGQWFFDLNTGGGSGG------------ PilC3_Ngo ILGINTADGGAL-TPRSARPIVPEANTAVAQYSGHKTTSKGKSIPIGCMEKGGKTVCPNG PilC4_Ngo ILGINTADGGAL-TPRSARPIVPGD--QVAQYSGHQKMN-GKSVPIGCMWKNGKTVCPNG pilC2_Nme ILGINTADGGKL-TKKSARPIVPEANTAVAQYSGHKQTAKGKSIPIGCMWKNNETVCPNG pilC1_Nme ILGINTADGGKL-TKKSARPIVPEANTAVAQYSGHKQTAKGKSIPIGCMWKNNETVSPNG PilC_Nme ILGINTADGGKL-TKKSARPIVPEANTAVAQYSGHKKGTNGKSIPIGCMQKSNEIVCPNG Orf_Nme ILGINTADGGKL-TKKSARPIVPAANSKVAQYSGDKKTSSGKSIPIGCMEKDGGTVCPNG PilC2_Ngo ILGINTADGGAL-TPRSARPIVPDHN-SVAQYSGHQKMN-GKSIPIGCMWKNSKTVCPNG PilC1_Ngo IVVINTADGGAL-TPRSARPIVPDHN-SVAQYSGHKKTAEGKSPPIGCMNKCGKTVCPNG Orf_Xfa FFSTYVPGVRVN-CASGGSNWLYVLDAASGGAALGQVNIPSRGSRSSMGNSDTGAVSTGG PilY1_Xcac FIPTYTPAVVSSGCKPDGANWLFGLDTRTGDGALMDARKGSPTGASFASRTAATPLTTSG ComC_Asp IYDPIAQDGRVADCSIGIQGLSSIRRYCLPYGVCEKQTDLSGMLKLGKG----------- . . PilY1a_Pae SYNKQNVAVSGTEQKGLGGLTLSTNEQGNPEVCSSGECLTVNPGPNTRGRQNWRPIEGKN PilY1b_Pae ----LEIRLTGTTRRNVG-----------------------NPVP---SRKAWEA----- PilY1a_Xfa VLNGNPVSGVGVSSAPNSPVFTGNIMQIGADDGTVTSLKTPSSGGLNINRVSWREILRTE PilY1b_Xfa ALNGKPVSGVGVSSAPNSPVFTGNIMQIGADDGTVTSLKTPSSGGLNINRVSWREILRP- PilC3_Ngo YVYDKPVNVRYLDETETDGFSTTADGDAGGSGIDPADRRPGKNNRCFSGKGVRTLLMNDL PilC4_Ngo YVYDKPVNVRYLDEKKTDDFPVTADGDAGGSGTFKEGKKPARNNRCFSGKGVRTLLMNDL pilC2_Nme YVYDKPVNVRYLDEKKTDGFSTTADGDAGGSGIDPAGKRSGKNNRCFSQKGVRTLLMNDL pilC1_Nme YVYDKPVNVRYLDEKKTDDFPVTADGDAGGSGTFKEGKKPARNNRCFSGKGVRTLLMNDL PilC_Nme YVYDKPVNVRYLDEKKTDGFSTTADGDAGGSGIDPAGKRSGKNNRCFSQKGVRTLLMNDL Orf_Nme YVYDKPVNVRYLDEKKTDGFSTTADGDAGGSGTFKEGKKPARNNRCFSGKGVRTLLMNDL PilC2_Ngo YVYDKPVNVRYLDEKKTDDFPVTADGDAGGSGTFKEGKKPARNNRCFSGKGVRTLLMNDL PilC1_Ngo YVDDKADIVRYLEKRKTDDFPVTADGDAGGSGTFKEGKKPARNNRCFSGKGVRTLLMNDL Orf_Xfa DAPIQSVAVTRTAPRQPIFCNPGEQGCFLVPEIPAAPLDTRCSEVIIDPNDPTRSISLFR PilY1_Xcac NAPVKDVGVSAIPRLSPAESGSAPGGQACWMVVTAAG-SGPLYIPYPCGRQSWRQVQ--- ComC_Asp -ILPVTIGSGSTDNKSTRQLIGGFSKDERTNAANVLGQNTLRRQIVPLKWYEQSTP---- PilY1a_Pae ---------------------- PilY1b_Pae ---------------------- PilY1a_Xfa ---------------------- PilY1b_Xfa ---------------------- PilC3_Ngo DSLDITGPMCGTKRLSWREVFF PilC4_Ngo DSLDITGPMCGIKRLSWREIFF pilC2_Nme DSLDITGPTCGMKRISWREVFF pilC1_Nme DSLDITGPTCGMKRISWREIFY PilC_Nme DSLDITGPTCGMKRISWREVFY Orf_Nme DSLDITGPMCGMKRISWREVFY PilC2_Ngo DSLDITGPMCGIKRLSWREVFF PilC1_Ngo DSLDITGPMCGIKRLSWREVFF Orf_Xfa ACGRQSWRQLR----------- PilY1_Xcac ---------------------- ComC_Asp ----------------------