Figures | |
| Figure S1: | Multiple alignment of all homologues in the SdpI family. The CLUSTALX program (Thompson et al., 1997) was used to generate this alignment. |
| Figure S2: | GAP alignment demonstrating the region of homology between varying topological types within SdpI family. Afu2 (residues 1 to 228), an inverted 6 TMS protein, is aligned with Tko1 (residues 1 to 237), an inverted 7 TMS representative. Quality: 413; Length: 240; Gaps: 8; Percent similarity: 54.2; Percent identity: 41.8. The average comparison score was 73.1 S.D. |
| Figure S3: | GAP alignment demonstrating the region of homology between varying topological types within SdpI family. Cdi2 (residues 106 to 200), a 6 TMS representative, is aligned with Cgl1 (residues 1 to 92), a 3 TMS representative. Quality: 79; Length: 95; Gaps: 2; Percent similarity: 41.3; Percent identity: 26.1. The average comparison score was 11.1 S.D. |
| Figure S4: | GAP alignment demonstrating the region of homology between varying topological types within SdpI family. Sin1 (residues 2 to 164), a 6 TMS representative, is aligned with Cte1 (residues 24 to 187), a 5 TMS Representative. Quality: 76; Length: 175; Gaps: 11; Percent similarity: 42.8; Percent identity: 30.9. The average comparison score was 12.4 S.D. |
| Figure S5: | GAP alignment demonstrating the region of homology between varying topological types within SdpI family. Bcl2 (from residues 1 to 164), a 5 TMS representative, is aligned with Mac1 (from 63 to 225 aa), a 6 TMS representative. Quality: 128; Length: 174; Gaps: 10; Percent similarity: 51.0; Percent identity: 34.6. The average comparison score was 21.3 S.D. |
| Figure S6: | GAP alignment demonstrating the region of homology between varying topological types within SdpI family. Ssa2 (residues 1 to 165), a 5 TMS representative, is aligned with Lpl1 (residues 1 to 198), a 6 TMS representative. Quality: 124; Length: 413; Gaps: 12; Percent similarity: 43.4; Percent identity: 33.8. The average comparison score was 17.2 S.D. |
| Figure S7: | GAP alignment demonstrating the region of homology between varying topological types within SdpI family. Rsa1 (residues 4 to 292), a 5 TMS representative, is aligned with Mac1 (residues 2 to 227), a 6 TMS representative. Quality: 58; Length: 299; Gaps: 7; Percent similarity: 33.8; Percent identity: 22.2. The average comparison score was 11.9 S.D. |
| Figure S8: | GAP alignment demonstrating the region of homology between varying topological types within SdpI family. Rsa1 (residues 1 to 292), a 5 TMS representative, is aligned with Lsp1 (residues 1 to 353), an 8 TMS representative. Quality: 174; Length: 417; Gaps: 10; Percent similarity: 41.2; Percent identity: 27.6. The average comparison score was 35.4 S.D. |
| Figure S9: | GAP alignment demonstrating the region of homology between varying topological types within SdpI family. Mac1 (residues 2 to 212), a 6 TMS representative, is aligned with Swo1 (residues 134 to 373), an 8 TMS representative. Quality: 98; Length: 243; Gaps: 13; Percent similarity: 42.3; Percent identity: 30.3. The average comparison score was 14.1 S.D. |
| Figure S10: | GAP alignment demonstrating the region of homology between varying topological types within SdpI family. Cpe1 (residues 1 to 199), a 6 TMS representative, is aligned with Dlo1 (residues 5 to 200), a 7 TMS representative. Quality: 144; Length: 209; Gaps: 9; Percent similarity: 45.2; Percent identity: 30.1. The average comparison score was 20.4 S.D. |
| Figure S11: | GAP alignment demonstrating the region of homology between varying topological types within SdpI family. Afu2 (residues 4 to 226), an inverted 6 TMS protein, is aligned with Dge1 (residues 92 to 338), a 12 TMS protein. Quality: 133; Length: 256; Gaps: 12; Percent similarity: 44.4; Percent identity: 34.6. The average comparison score was 20.9 S.D. |
| Figure S12: | Multiple alignment of the SdpI family homologues excluding the four inverted proteins and with the 12 TMS protein, Dge1 cut in half. The CLUSTALX program (Thompson et al., 1997) was used to generate this alignment. This multiple alignment was used to generate the AveHAS figure in Figure 10. |
| Figure S13: | Figure S13: The locations of the two best-conserved motifs for the SdpI family proteins analyzed in this study, arranged by cluster and generated with the MEME program (Bailey, TL., Elkan, C. 1995). Contrary to our final conclusions, this figure depicts Swo1 and Cac2, both 8 TMS proteins, as containing Motif 1. However, the p-value is poor for this motif in both proteins: 9.34e-05 for Cac2 and 7.45e-06 for Swo1, suggesting low statistical significance. This motif was not found upon manual examination of the sequence or GAP analysis in either protein. Similarly, Dge1 shows a repeat of Motif 2 at the end of the sequence, but its p-value of 6.77e-05 is also poor. Motif 2 was not found in this location using manual sequence examination or GAP analysis. These motifs could be false positives. |
